Detailed information of TA system TA066257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 7989..8907
• Replicon: plasmid pFORC46.2
• Accession: NZ_CP017231
• Organism: Campylobacter jejuni strain FORC_046 isolate Not reported

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: A0A5T1Y6U9
• Locus tag: FORC46_RS08830
• Protein ID: WP_019109036.1
• Coordinates: 8224..8907 (+)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: A0A3Z9GMV3
• Locus tag: FORC46_RS08825
• Protein ID: WP_002907823.1
• Coordinates: 7989..8231 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC46_RS08810	3128..5341	+	2214	WP_052796197.1	DUF1738 domain-containing protein	-
FORC46_RS08815	5338..7161	+	1824	WP_052796196.1	type IV secretory system conjugative DNA transfer family protein	-
FORC46_RS08820	7178..7897	+	720	WP_052796195.1	conjugal transfer protein TraL	-
FORC46_RS08825	7989..8231	+	243	WP_002907823.1	hypothetical protein	Antitoxin
FORC46_RS08830	8224..8907	+	684	WP_019109036.1	Fic family protein	Toxin
FORC46_RS08835	8912..9379	+	468	WP_002907837.1	signal peptidase I	-
FORC46_RS08840	9468..10109	+	642	WP_088868052.1	DNA/RNA non-specific endonuclease	-
FORC46_RS08845	10142..10537	+	396	WP_052796193.1	conjugal transfer protein TraM	-
FORC46_RS08850	10618..11304	+	687	WP_002907859.1	AAA family ATPase	-
FORC46_RS08855	11307..11531	+	225	WP_052796192.1	hypothetical protein	-
FORC46_RS08860	11878..12432	+	555	WP_079460393.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28521	28521

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 26138.30 Da
• Isoelectric Point: 10.1130

>T66257 WP_019109036.1 NZ_CP017231:8224-8907 [Campylobacter jejuni]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKREREITLKKQLKLQLNPIKGNFDYQHLKDIHKALFEDVYTWA
GQDRMQMGLKEKFAKYAPNGAIINFVPGKELDKYSKIIFDELAKNNYLKNSKDLNHFAKNLAKFMGEINALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLISKHKMIHACAEASQLKPGRLEALIKDNLKNFRQNLDLEQNKGISL

Nucleotide

• Length: 684 bp

>T66257 NZ_CP017231:8224-8907 [Campylobacter jejuni]
ATGATTGATAAAGTTTTGAAATTTTTAGATGATGGTATTCCTAAAAATGATTTTGACAGACAACTTTTAGCTTCTAATTT
AGTAGGCGCAACAAATTTAATAGAGTTAAATAAACGAGAAAGAGAAATTACATTAAAAAAACAATTAAAACTACAACTCA
ATCCCATTAAAGGCAATTTCGATTATCAGCATTTAAAAGATATACATAAAGCACTTTTTGAAGATGTTTATACTTGGGCA
GGACAAGATAGAATGCAAATGGGACTAAAAGAAAAATTTGCAAAATATGCACCCAATGGAGCAATTATTAATTTTGTTCC
TGGAAAAGAGTTAGATAAATACTCTAAAATAATTTTTGATGAACTTGCAAAAAATAACTATCTAAAAAACTCCAAAGATC
TAAATCATTTTGCAAAAAATTTAGCAAAATTTATGGGCGAAATCAATGCTTTACACCCTTTTAGAGAAGGTAACGGCAGA
ACTCAAAGAATATTTTTAAACGAGTTGGCTAAAAATGCAGGTTATAAACTTGATTTAAATTTAATTTCTAAGCATAAAAT
GATACATGCTTGTGCAGAAGCTTCACAATTAAAACCTGGAAGATTGGAGGCTTTAATTAAAGACAATCTAAAAAACTTTA
GACAAAATTTAGATTTGGAGCAAAATAAAGGAATATCATTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9276.49 Da
• Isoelectric Point: 4.3697

>AT66257 WP_002907823.1 NZ_CP017231:7989-8231 [Campylobacter jejuni]
MQKQYPEVHSLEESLAILKKYKDDLTKEQYEGIKSVICGHAIEDMFANEKDVIDMIKIAKDEANADEIIAEYKREWGVND

Nucleotide

• Length: 243 bp

>AT66257 NZ_CP017231:7989-8231 [Campylobacter jejuni]
ATGCAAAAACAATATCCCGAAGTGCATAGCTTAGAAGAAAGTCTTGCGATACTTAAAAAATATAAAGATGATTTAACTAA
AGAGCAATATGAAGGTATTAAATCTGTTATTTGCGGACACGCTATTGAAGATATGTTTGCAAATGAAAAAGATGTTATCG
ATATGATAAAAATTGCAAAAGATGAAGCAAATGCCGATGAAATAATCGCCGAATATAAAAGAGAATGGGGAGTAAATGAT
TGA

Structures

Toxin

• AlphaFold DB: A0A5T1Y6U9

Antitoxin

• AlphaFold DB: A0A3Z9GMV3