Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 122568..123093 Replicon plasmid pFAM21845_1
Accession NZ_CP017221
Organism Escherichia coli strain FAM21845

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BHT24_RS26625 Protein ID WP_001159871.1
Coordinates 122568..122873 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag BHT24_RS26630 Protein ID WP_000813630.1
Coordinates 122875..123093 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT24_RS26610 118530..119696 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
BHT24_RS26615 120284..121039 - 756 WP_000852146.1 replication initiation protein RepE -
BHT24_RS26620 121761..122567 - 807 WP_000016970.1 site-specific integrase -
BHT24_RS26625 122568..122873 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BHT24_RS26630 122875..123093 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BHT24_RS26645 123721..123951 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BHT24_RS26650 123948..124364 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
BHT24_RS26655 124439..126004 + 1566 WP_001128474.1 AAA family ATPase -
BHT24_RS26660 125989..127011 + 1023 WP_000361402.1 helicase UvrD -
BHT24_RS26670 127265..127951 - 687 Protein_151 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / dfrA1 / ant(3'')-Ia / qacE / sul1 / blaTEM-1A / sitABCD iutA / iucD / iucC / iucB / iucA 1..147225 147225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T66254 WP_001159871.1 NZ_CP017221:c122873-122568 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T66254 NZ_CP017221:c122873-122568 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT66254 WP_000813630.1 NZ_CP017221:c123093-122875 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT66254 NZ_CP017221:c123093-122875 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References