Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4816035..4816611 Replicon chromosome
Accession NZ_CP017220
Organism Escherichia coli strain FAM21845

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag BHT24_RS25285 Protein ID WP_001297643.1
Coordinates 4816324..4816611 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag BHT24_RS25280 Protein ID WP_000063156.1
Coordinates 4816035..4816337 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT24_RS25265 4812631..4814781 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
BHT24_RS25270 4814831..4815034 + 204 WP_000467859.1 YbdD/YjiX family protein -
BHT24_RS25275 4815045..4816001 + 957 WP_001297640.1 GTPase -
BHT24_RS25280 4816035..4816337 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
BHT24_RS25285 4816324..4816611 - 288 WP_001297643.1 BrnT family toxin Toxin
BHT24_RS25290 4816810..4817507 - 698 Protein_4609 IS1 family transposase -
BHT24_RS25295 4817558..4819846 - 2289 WP_057107720.1 phosphatidylglycerol--membrane-oligosaccharide glycerophosphotransferase -
BHT24_RS25300 4820100..4820594 - 495 WP_077786330.1 DUF2501 domain-containing protein -
BHT24_RS25305 4820643..4821380 - 738 WP_000799911.1 DNA replication protein DnaC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4814831..4870019 55188


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T66244 WP_001297643.1 NZ_CP017220:c4816611-4816324 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T66244 NZ_CP017220:c4816611-4816324 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT66244 WP_000063156.1 NZ_CP017220:c4816337-4816035 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT66244 NZ_CP017220:c4816337-4816035 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References