Detailed information of TA system TA066230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1723004..1723530
• Replicon: chromosome
• Accession: NZ_CP017220
• Organism: Escherichia coli strain FAM21845

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: BHT24_RS08955
• Protein ID: WP_000323025.1
• Coordinates: 1723004..1723291 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: BHT24_RS08960
• Protein ID: WP_000534858.1
• Coordinates: 1723291..1723530 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHT24_RS08915	1718028..1718243	-	216	WP_000839590.1	phage lysis protein EssD	-
BHT24_RS28180	1718463..1718633	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
BHT24_RS08920	1718997..1719212	-	216	WP_000066484.1	cold shock-like protein CspB	-
BHT24_RS08925	1719513..1719725	+	213	WP_000087756.1	cold shock-like protein CspF	-
BHT24_RS27405	1719780..1719869	+	90	WP_120795389.1	hypothetical protein	-
BHT24_RS08930	1720147..1720899	-	753	WP_001047135.1	antitermination protein	-
BHT24_RS08935	1720913..1721962	-	1050	WP_028985484.1	DUF968 domain-containing protein	-
BHT24_RS08940	1721964..1722242	-	279	WP_012304870.1	hypothetical protein	-
BHT24_RS08945	1722309..1722560	-	252	WP_000980994.1	hypothetical protein	-
BHT24_RS08950	1722777..1722932	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
BHT24_RS08955	1723004..1723291	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
BHT24_RS08960	1723291..1723530	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
BHT24_RS08990	1725266..1725963	+	698	Protein_1647	IS1 family transposase	-
BHT24_RS08995	1725980..1726831	-	852	Protein_1648	Zn-dependent oxidoreductase	-
BHT24_RS09000	1726843..1728057	-	1215	WP_001295394.1	starvation-sensing protein RspA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1685613..1738692	53079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T66230 WP_000323025.1 NZ_CP017220:c1723291-1723004 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T66230 NZ_CP017220:c1723291-1723004 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT66230 WP_000534858.1 NZ_CP017220:c1723530-1723291 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT66230 NZ_CP017220:c1723530-1723291 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8