Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 562488..563106 Replicon chromosome
Accession NZ_CP017220
Organism Escherichia coli strain FAM21845

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BHT24_RS03005 Protein ID WP_001291435.1
Coordinates 562488..562706 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BHT24_RS03010 Protein ID WP_000344800.1
Coordinates 562732..563106 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHT24_RS02970 557777..558349 + 573 WP_000779831.1 YbaY family lipoprotein -
BHT24_RS02975 558380..558691 - 312 WP_000409911.1 MGMT family protein -
BHT24_RS02985 559070..559423 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BHT24_RS02990 559465..561015 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BHT24_RS02995 561179..561649 - 471 WP_000136192.1 YlaC family protein -
BHT24_RS03000 561765..562316 - 552 WP_000102564.1 maltose O-acetyltransferase -
BHT24_RS03005 562488..562706 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BHT24_RS03010 562732..563106 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BHT24_RS03020 563270..563967 + 698 Protein_563 IS1 family transposase -
BHT24_RS03025 564429..567578 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 563590..563967 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T66227 WP_001291435.1 NZ_CP017220:c562706-562488 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T66227 NZ_CP017220:c562706-562488 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT66227 WP_000344800.1 NZ_CP017220:c563106-562732 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT66227 NZ_CP017220:c563106-562732 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References