Detailed information of TA system TA066226

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 313503..314182
• Replicon: chromosome
• Accession: NZ_CP017220
• Organism: Escherichia coli strain FAM21845

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: BHT24_RS01590
• Protein ID: WP_000854680.1
• Coordinates: 313503..313844 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: BHT24_RS01595
• Protein ID: WP_000070396.1
• Coordinates: 313865..314182 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHT24_RS01565	308744..309058	-	315	WP_000951129.1	hypothetical protein	-
BHT24_RS28140	309220..309276	+	57	WP_211180520.1	protein YsgD	-
BHT24_RS01570	309428..310378	+	951	WP_000947155.1	magnesium/cobalt transporter CorA	-
BHT24_RS01575	310532..311218	+	687	Protein_295	IS1 family transposase	-
BHT24_RS01580	311230..311901	-	672	Protein_296	hypothetical protein	-
BHT24_RS01585	311911..312984	-	1074	WP_001396517.1	site-specific integrase	-
BHT24_RS01590	313503..313844	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
BHT24_RS01595	313865..314182	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
BHT24_RS01600	314201..314422	-	222	WP_000691994.1	DUF987 domain-containing protein	-
BHT24_RS01605	314431..314907	-	477	WP_000811693.1	RadC family protein	-
BHT24_RS01610	314923..315381	-	459	WP_000211838.1	antirestriction protein	-
BHT24_RS01615	315479..315718	-	240	WP_000194654.1	DUF905 domain-containing protein	-
BHT24_RS01620	315795..316262	-	468	WP_057107658.1	hypothetical protein	-
BHT24_RS01625	316286..316729	-	444	WP_016239438.1	phage transcriptional regulator	-
BHT24_RS01630	316729..316965	-	237	WP_057107659.1	hypothetical protein	-
BHT24_RS01635	317006..317707	-	702	WP_023326104.1	WYL domain-containing protein	-
BHT24_RS01640	317924..318745	-	822	WP_001547762.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T66226 WP_000854680.1 NZ_CP017220:c313844-313503 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T66226 NZ_CP017220:c313844-313503 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT66226 WP_000070396.1 NZ_CP017220:c314182-313865 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT66226 NZ_CP017220:c314182-313865 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7