Detailed information of TA system TA066223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 798293..798768
• Replicon: chromosome
• Accession: NZ_CP017196
• Organism: Leuconostoc gelidum subsp. gelidum strain TMW 2.1618

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BHS02_RS04060
• Protein ID: WP_174249137.1
• Coordinates: 798293..798553 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BHS02_RS04065
• Protein ID: WP_090090295.1
• Coordinates: 798553..798768 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHS02_RS04010	794334..794711	+	378	WP_174249129.1	hypothetical protein	-
BHS02_RS04015	794698..794949	+	252	WP_174249130.1	hypothetical protein	-
BHS02_RS04020	795219..795512	-	294	WP_174248743.1	hypothetical protein	-
BHS02_RS04025	795530..795823	-	294	WP_174249131.1	hypothetical protein	-
BHS02_RS04030	795839..796189	-	351	WP_174249132.1	hypothetical protein	-
BHS02_RS04035	796164..796406	-	243	WP_174249133.1	hypothetical protein	-
BHS02_RS04040	796773..796991	-	219	Protein_740	hypothetical protein	-
BHS02_RS04045	797006..797218	-	213	WP_174249134.1	hypothetical protein	-
BHS02_RS04050	797211..797654	-	444	WP_174249135.1	hypothetical protein	-
BHS02_RS04055	797651..797878	-	228	WP_174249136.1	hypothetical protein	-
BHS02_RS04060	798293..798553	-	261	WP_174249137.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BHS02_RS04065	798553..798768	-	216	WP_090090295.1	CopG family transcriptional regulator	Antitoxin
BHS02_RS04070	798833..799039	-	207	WP_174249138.1	hypothetical protein	-
BHS02_RS04075	799021..799398	-	378	WP_174249139.1	hypothetical protein	-
BHS02_RS04080	799512..799943	-	432	WP_174249140.1	JAB domain-containing protein	-
BHS02_RS04085	800402..800668	+	267	WP_174249141.1	hypothetical protein	-
BHS02_RS04090	800655..802910	+	2256	WP_174249142.1	toprim domain-containing protein	-
BHS02_RS04095	802926..803408	+	483	WP_174249143.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Protein of unknown function DUF6290 (1-69)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10126.53 Da
• Isoelectric Point: 9.7391

>T66223 WP_174249137.1 NZ_CP017196:c798553-798293 [Leuconostoc gelidum subsp. gelidum]
MYNVTFTKQALKQLKKLDKFTQSTIYQWIVNNLDGTDNPRLHGKGLTANHSGQWRYRVGDYRVLANIIDDEIVIEIFTIG
HRRDIY

Nucleotide

• Length: 261 bp

>T66223 NZ_CP017196:c798553-798293 [Leuconostoc gelidum subsp. gelidum]
ATGTATAACGTTACTTTTACCAAACAGGCTTTAAAACAATTAAAAAAATTAGATAAATTTACACAGAGCACAATTTACCA
ATGGATTGTCAATAACCTTGATGGCACTGATAACCCGCGACTGCATGGCAAAGGATTAACAGCTAATCATTCTGGACAAT
GGCGTTATCGTGTCGGAGATTATCGTGTATTAGCCAATATTATTGACGACGAAATTGTGATTGAAATATTTACTATTGGA
CATCGTCGAGATATTTATTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8037.18 Da
• Isoelectric Point: 4.5580

>AT66223 WP_090090295.1 NZ_CP017196:c798768-798553 [Leuconostoc gelidum subsp. gelidum]
MTTMTLRINQDDSELIKKFAATHDLSLSDFARQAMIEKIEDDYDLAALRKAMAEDDGTRYTLAEIKKALAL

Nucleotide

• Length: 216 bp

>AT66223 NZ_CP017196:c798768-798553 [Leuconostoc gelidum subsp. gelidum]
ATGACAACTATGACTTTACGTATCAATCAAGATGATTCAGAGCTTATTAAAAAATTTGCTGCAACACACGACTTGAGCTT
GTCAGATTTTGCGCGTCAGGCGATGATTGAAAAAATTGAAGATGACTATGACCTCGCTGCACTTCGTAAAGCTATGGCAG
AAGATGATGGCACACGTTACACGCTGGCCGAAATAAAAAAGGCTTTGGCATTATAA