Detailed information of TA system TA066222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 21431..21966
• Replicon: plasmid p_XCV_2
• Accession: NZ_CP017192
• Organism: Xanthomonas campestris pv. vesicatoria str. 85-10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3C0B3
• Locus tag: BHE83_RS23390
• Protein ID: WP_007974210.1
• Coordinates: 21646..21966 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3C0B2
• Locus tag: BHE83_RS23385
• Protein ID: WP_011345582.1
• Coordinates: 21431..21646 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHE83_RS23355	17636..17866	-	231	WP_046935364.1	hypothetical protein	-
BHE83_RS23360	17953..18915	-	963	WP_011345584.1	recombinase family protein	-
BHE83_RS23365	19074..19760	-	687	WP_007968793.1	DNA-binding protein	-
BHE83_RS23375	20236..20862	+	627	WP_007974205.1	ParA family protein	-
BHE83_RS23380	20859..21185	+	327	WP_007974207.1	hypothetical protein	-
BHE83_RS23385	21431..21646	+	216	WP_011345582.1	antitoxin MazE family protein	Antitoxin
BHE83_RS23390	21646..21966	+	321	WP_007974210.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BHE83_RS23395	22635..23453	-	819	WP_011345581.1	hypothetical protein	-
BHE83_RS23400	23959..24288	-	330	WP_007967027.1	hypothetical protein	-
BHE83_RS23405	24792..25073	+	282	WP_041854934.1	hypothetical protein	-
BHE83_RS23410	25102..25479	+	378	WP_011345609.1	TrbC/VirB2 family protein	-
BHE83_RS23415	25476..25775	+	300	WP_041854933.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38116	38116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11571.48 Da
• Isoelectric Point: 6.2890

>T66222 WP_007974210.1 NZ_CP017192:21646-21966 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MQRGDLVTVSLQGDYGKPRPALVVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Nucleotide

• Length: 321 bp

>T66222 NZ_CP017192:21646-21966 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGCAGCGGGGCGACCTTGTGACGGTTTCATTGCAGGGCGACTATGGCAAACCACGGCCCGCCTTGGTCGTTCAATCCGA
CTTGCTCACCGACCTGGAAAGCGTCGTGCTGTGCCCGGTGACAAGCGACCTGCGCAATGCCGCGTTCCGCGTCACCGTGG
AACCGAACCCGGCCAACGGGCTGCGCGCGCTCTCGCAGGTCATGGTGGACAAGCTCTCAACGCTGCCTCGCACCAAGATC
AGCGAACCGTTTGGCCGCCTCGATGATGAGCGTATGAAAGCGATAGACAGGGCGCTCCTGCTGGTCGTTGGCGTTATCTG
A

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8190.20 Da
• Isoelectric Point: 7.2801

>AT66222 WP_011345582.1 NZ_CP017192:21431-21646 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MSSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFAAQVRKQCQNLKGDPAETEVLRFTEEAATHVEGWE

Nucleotide

• Length: 216 bp

>AT66222 NZ_CP017192:21431-21646 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGTCATCAATTCACGAATCAAAGGTAGCCCGGCACCGCGAGCGGATGCGCGCTGCGGGGCTACGGCCTGTCCAGTTTTG
GGTGCCCGACACACGCTCTCCCGAGTTCGCGGCGCAAGTGCGCAAGCAGTGCCAAAACCTGAAGGGCGACCCGGCAGAAA
CCGAGGTTTTGCGCTTCACCGAAGAGGCCGCAACGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: Q3C0B3

Antitoxin

• AlphaFold DB: Q3C0B2