Detailed information of TA system TA066221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4994..5535
• Replicon: plasmid p_XCV_2
• Accession: NZ_CP017192
• Organism: Xanthomonas campestris pv. vesicatoria str. 85-10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BHE83_RS23280
• Protein ID: WP_007968778.1
• Coordinates: 5209..5535 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7W9EDN7
• Locus tag: BHE83_RS23275
• Protein ID: WP_033478260.1
• Coordinates: 4994..5212 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHE83_RS23245	792..1814	+	1023	WP_007968768.1	P-type DNA transfer ATPase VirB11	-
BHE83_RS23250	1825..2712	+	888	WP_041854937.1	lytic transglycosylase domain-containing protein	-
BHE83_RS23255	2758..3345	+	588	WP_011345600.1	hypothetical protein	-
BHE83_RS23260	3388..3963	+	576	WP_041854932.1	phospholipase D family protein	-
BHE83_RS23265	3974..4387	+	414	WP_041854931.1	hypothetical protein	-
BHE83_RS23270	4441..4857	+	417	WP_017159868.1	hypothetical protein	-
BHE83_RS23275	4994..5212	+	219	WP_033478260.1	antitoxin MazE family protein	Antitoxin
BHE83_RS23280	5209..5535	+	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BHE83_RS23285	5605..8580	-	2976	WP_011345597.1	conjugative relaxase	-
BHE83_RS23290	8594..10168	-	1575	WP_011345596.1	type IV secretion system DNA-binding domain-containing protein	-
BHE83_RS23295	10172..10369	-	198	WP_011345595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38116	38116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T66221 WP_007968778.1 NZ_CP017192:5209-5535 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T66221 NZ_CP017192:5209-5535 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8476.58 Da
• Isoelectric Point: 5.0742

>AT66221 WP_033478260.1 NZ_CP017192:4994-5212 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT66221 NZ_CP017192:4994-5212 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7W9EDN7