Detailed information of TA system TA066220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 133447..134105
• Replicon: plasmid p_XCV_1
• Accession: NZ_CP017191
• Organism: Xanthomonas campestris pv. vesicatoria str. 85-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: BHE83_RS22995
• Protein ID: WP_011053002.1
• Coordinates: 133704..134105 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: BHE83_RS22990
• Protein ID: WP_005917852.1
• Coordinates: 133447..133707 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHE83_RS22980	129223..129645	+	423	Protein_129	resolvase	-
BHE83_RS26035	129921..130991	+	1071	WP_011345706.1	tyrosine phosphatase	-
BHE83_RS25360	131082..132419	+	1338	WP_011037255.1	avirulence protein	-
BHE83_RS22985	132691..133257	-	567	WP_011345705.1	recombinase family protein	-
BHE83_RS22990	133447..133707	+	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BHE83_RS22995	133704..134105	+	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
BHE83_RS23000	134130..134393	+	264	WP_005917846.1	hypothetical protein	-
BHE83_RS23005	134400..135587	+	1188	WP_011053001.1	zeta toxin family protein	-
BHE83_RS23010	135867..137093	+	1227	WP_011345704.1	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..182571	182571
	-	inside	IScluster/Tn	-	-	118366..145879	27513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T66220 WP_011053002.1 NZ_CP017191:133704-134105 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T66220 NZ_CP017191:133704-134105 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGAAGCGGTACATGCTTGATACCAACACCGTGAGCCATCTCGTCAAGAGCCATCCGGCTGTCTCTCGACGGGTCATTGA
AGTGCCTATGACTGCGCTATGTATGTCCGCAATTACCGGTGGGGAGCTGATGTTCGGCTTGGCTAAGGTGCCAGACGCCA
AACGCCTTCAGCAAGCCGTCATGGAACTCCTGCGGCGCGTGGATGTGCTGCCTTGGGATGGCGCGGTAATGGAGCGTTAC
GGAAGCGTAAGAGCGGACCTAGAAAAGCAAGGTAAAGCGCTTGGGTCGCTCGACATGCTTATCGCAGCACATGCACTGGA
AACGGATTCTGTCCTCGTTACCAATGACGCCGCATTTAGTCGTGTTGTTGGTTTGACCGTGGAAGATTGGACACGCAGCT
AA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT66220 WP_005917852.1 NZ_CP017191:133447-133707 [Xanthomonas campestris pv. vesicatoria str. 85-10]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT66220 NZ_CP017191:133447-133707 [Xanthomonas campestris pv. vesicatoria str. 85-10]
GTGAGCCAAGTTGCCAAGCTGTTCATCAATGGCCGCAGCCAGGCAGTCCGCTTGCCTGCCGCCTATCGCTTTACCGAGAA
AGAGGTTTTCATTCGCCAAGACCCGGAGACCGGTGACGTCATCCTGTCTCGGAAGCCTGCAACGTGGGAGGACTTCTTTA
TCGCACTCAAGAGCGCAGATGTGCCTGCTAATTTTCTAGATGCCGCCGAGCGCAATCAGGGCACGCAGGACCGTGATCCT
TTTGCGGGATGGCGCGAATGA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002