Detailed information of TA system TA066213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 46667..47292
• Replicon: plasmid pDSMZ14563
• Accession: NZ_CP017187
• Organism: Enterobacter hormaechei subsp. hoffmannii strain DSM 14563

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BFV65_RS21670
• Protein ID: WP_069597122.1
• Coordinates: 46894..47292 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BFV65_RS21665
• Protein ID: WP_053065088.1
• Coordinates: 46667..46894 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV65_RS21650	42516..43979	-	1464	WP_047732321.1	family 1 glycosylhydrolase	-
BFV65_RS21655	43979..45790	-	1812	WP_047732316.1	beta-glucoside-specific PTS transporter subunit IIABC	-
BFV65_RS21660	46244..46588	-	345	Protein_39	transposase	-
BFV65_RS21665	46667..46894	+	228	WP_053065088.1	antitoxin	Antitoxin
BFV65_RS21670	46894..47292	+	399	WP_069597122.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BFV65_RS21675	47289..47651	-	363	WP_047732307.1	hypothetical protein	-
BFV65_RS23245	48341..48583	+	243	WP_142761956.1	multidrug effflux MFS transporter	-
BFV65_RS21680	49024..49281	+	258	WP_077258044.1	DUF2767 family protein	-
BFV65_RS21685	49534..49695	-	162	WP_047732281.1	type I toxin-antitoxin system Hok family toxin	-
BFV65_RS21690	50610..52046	+	1437	WP_053065155.1	coniferyl aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131604	131604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14802.14 Da
• Isoelectric Point: 9.5569

>T66213 WP_069597122.1 NZ_CP017187:46894-47292 [Enterobacter hormaechei subsp. hoffmannii]
MLRYMLDTNICIFTIKNKPEAVRVKFNLHRQRLCISTITLMELIYGAEKSSAPERNLAVVEGFAARLTVLDYDAHAATHT
GQIRAEQAKAGQPIGPYVQMIAGHARSQGLIVVTNNMREFSRVSGLRSEDWI

Nucleotide

• Length: 399 bp

>T66213 NZ_CP017187:46894-47292 [Enterobacter hormaechei subsp. hoffmannii]
ATGCTCAGGTACATGCTGGACACCAACATCTGCATTTTCACCATCAAAAATAAACCGGAAGCCGTCAGGGTGAAGTTCAA
CCTTCACCGCCAGCGGCTTTGCATCAGCACCATCACGCTGATGGAGTTGATATATGGCGCGGAGAAAAGCAGCGCACCGG
AACGCAATCTGGCCGTGGTGGAAGGCTTCGCCGCCCGCCTGACCGTCCTGGATTACGATGCGCACGCCGCCACGCACACC
GGGCAAATCCGCGCTGAACAGGCAAAAGCCGGTCAGCCCATTGGTCCATACGTCCAGATGATTGCCGGTCATGCCCGCAG
TCAGGGCCTCATTGTTGTCACCAACAATATGAGGGAGTTTTCCCGCGTGTCCGGCCTTCGGTCCGAAGACTGGATTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8394.35 Da
• Isoelectric Point: 4.3558

>AT66213 WP_053065088.1 NZ_CP017187:46667-46894 [Enterobacter hormaechei subsp. hoffmannii]
METTVFLSNRSQAVRLPKAVALPDDVKHVEIVAVGRSRIISPAGESWDAWFDGDAVTSDFMSTREQPDTQIREDF

Nucleotide

• Length: 228 bp

>AT66213 NZ_CP017187:46667-46894 [Enterobacter hormaechei subsp. hoffmannii]
ATGGAAACGACAGTATTTTTAAGTAACCGGAGTCAGGCCGTGCGCCTGCCAAAAGCAGTCGCACTGCCGGATGATGTAAA
ACATGTTGAGATTGTGGCCGTTGGCCGGAGCCGCATCATTTCACCCGCAGGTGAGTCCTGGGATGCGTGGTTTGATGGCG
ACGCGGTGACGTCAGACTTTATGTCAACACGCGAGCAGCCCGACACACAGATACGGGAAGACTTCTGA