Detailed information of TA system TA066212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 33677..34314
• Replicon: plasmid pDSMZ14563
• Accession: NZ_CP017187
• Organism: Enterobacter hormaechei subsp. hoffmannii strain DSM 14563

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BFV65_RS21605
• Protein ID: WP_063136850.1
• Coordinates: 33904..34314 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: BFV65_RS21600
• Protein ID: WP_047732352.1
• Coordinates: 33677..33907 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV65_RS21585	28836..29420	+	585	WP_023345591.1	TetR/AcrR family transcriptional regulator	-
BFV65_RS21590	29488..30330	+	843	WP_048291887.1	nitroreductase family protein	-
BFV65_RS21595	30804..33014	+	2211	WP_063136849.1	AAA family ATPase	-
BFV65_RS21600	33677..33907	+	231	WP_047732352.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BFV65_RS21605	33904..34314	+	411	WP_063136850.1	type II toxin-antitoxin system VapC family toxin	Toxin
BFV65_RS21610	34460..35530	-	1071	WP_130941442.1	hypothetical protein	-
BFV65_RS21615	36321..36950	-	630	WP_063136851.1	DUF1349 domain-containing protein	-
BFV65_RS21620	37032..38213	-	1182	WP_063136852.1	MFS transporter	-
BFV65_RS21625	38247..39005	-	759	WP_053065089.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131604	131604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14806.04 Da
• Isoelectric Point: 5.1871

>T66212 WP_063136850.1 NZ_CP017187:33904-34314 [Enterobacter hormaechei subsp. hoffmannii]
VNRIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAELSQAAKESGPGTQNLADAFAARLDAILPWDRAAV
DATTEIKVALRMAGTPIDPNDTAIAGHAIAACAVLVTNNVREFERVPGLALEDWVK

Nucleotide

• Length: 411 bp

>T66212 NZ_CP017187:33904-34314 [Enterobacter hormaechei subsp. hoffmannii]
GTGAACAGGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGCATCGTGGTCTCGGCCATCACCTGGGCCGAGCTGTCACAGGCAGCGAAGGAGT
CCGGTCCCGGCACGCAGAATCTGGCTGACGCCTTCGCCGCACGCCTCGATGCCATCCTGCCCTGGGACCGCGCCGCGGTG
GATGCGACCACGGAGATTAAGGTCGCGCTGCGCATGGCCGGGACGCCGATCGACCCGAACGACACGGCGATTGCCGGGCA
CGCCATCGCCGCCTGCGCCGTGCTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGGCCTGGCACTGGAAGACT
GGGTTAAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.83 Da
• Isoelectric Point: 4.4692

>AT66212 WP_047732352.1 NZ_CP017187:33677-33907 [Enterobacter hormaechei subsp. hoffmannii]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIIREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT66212 NZ_CP017187:33677-33907 [Enterobacter hormaechei subsp. hoffmannii]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATATGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCATCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGTTCGTTCGCGCAGCTCG
AAAAAGCCGACCCGGACTTTATGGCAGAGCGCGAGGATGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA