Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1064197..1064818 Replicon chromosome
Accession NZ_CP017186
Organism Enterobacter hormaechei subsp. hoffmannii strain DSM 14563

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag BFV65_RS04960 Protein ID WP_015571250.1
Coordinates 1064197..1064415 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag BFV65_RS04965 Protein ID WP_006809850.1
Coordinates 1064444..1064818 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV65_RS04930 1060209..1060469 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
BFV65_RS04935 1060472..1060612 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BFV65_RS04940 1060609..1061319 - 711 WP_069596306.1 GNAT family N-acetyltransferase -
BFV65_RS04945 1061422..1062882 + 1461 WP_069596307.1 PLP-dependent aminotransferase family protein -
BFV65_RS04950 1062854..1063321 - 468 WP_023305595.1 hypothetical protein -
BFV65_RS04955 1063438..1063989 - 552 WP_023305596.1 maltose O-acetyltransferase -
BFV65_RS04960 1064197..1064415 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
BFV65_RS04965 1064444..1064818 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
BFV65_RS04970 1065328..1068474 - 3147 WP_023299464.1 multidrug efflux RND transporter permease subunit -
BFV65_RS04975 1068497..1069690 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T66200 WP_015571250.1 NZ_CP017186:c1064415-1064197 [Enterobacter hormaechei subsp. hoffmannii]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T66200 NZ_CP017186:c1064415-1064197 [Enterobacter hormaechei subsp. hoffmannii]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT66200 WP_006809850.1 NZ_CP017186:c1064818-1064444 [Enterobacter hormaechei subsp. hoffmannii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT66200 NZ_CP017186:c1064818-1064444 [Enterobacter hormaechei subsp. hoffmannii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References