Detailed information of TA system TA066187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1529914..1530827
• Replicon: chromosome
• Accession: NZ_CP017184
• Organism: Enterobacter roggenkampii strain DSM 16690

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A8B2SFJ4
• Locus tag: BFV67_RS07075
• Protein ID: WP_045334129.1
• Coordinates: 1529914..1530387 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: BFV67_RS07080
• Protein ID: WP_069598066.1
• Coordinates: 1530384..1530827 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV67_RS07055	1526074..1527384	+	1311	WP_069598063.1	type I-F CRISPR-associated protein Csy1	-
BFV67_RS07060	1527381..1528316	+	936	WP_069598064.1	type I-F CRISPR-associated protein Csy2	-
BFV67_RS07065	1528330..1529328	+	999	WP_023327014.1	type I-F CRISPR-associated protein Csy3	-
BFV67_RS07070	1529338..1529892	+	555	WP_069598065.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
BFV67_RS07075	1529914..1530387	-	474	WP_045334129.1	RES domain-containing protein	Toxin
BFV67_RS07080	1530384..1530827	-	444	WP_069598066.1	DUF2384 domain-containing protein	Antitoxin
BFV67_RS07085	1532166..1532384	-	219	WP_002211347.1	translation initiation factor IF-1	-
BFV67_RS07090	1532669..1533373	-	705	WP_008499991.1	leucyl/phenylalanyl-tRNA--protein transferase	-
BFV67_RS07095	1533419..1535140	-	1722	WP_069598067.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (15-147)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17539.85 Da
• Isoelectric Point: 5.0700

>T66187 WP_045334129.1 NZ_CP017184:c1530387-1529914 [Enterobacter roggenkampii]
MIFYRLVTGRYASEAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVRKDTVLNQYQLFSIDIPDDQIDYLDKA
WLPEDWQENPAPVSTMDLGTGWLQANSALALILPSCIIPYENNAILNPLHPAFHSALNSVQQLPFIFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T66187 NZ_CP017184:c1530387-1529914 [Enterobacter roggenkampii]
ATGATTTTTTATCGTCTGGTGACAGGACGCTATGCCAGCGAGGCGTGGAGTGGGAGTGGTGCAAACCAGTATGGCGGACG
GTGGAATCATAAAGGCCATCCAGCTGTGTATGTCTCCACCTCCATCTCGCTGGCCTCACTGGAGATTTTGGTACATGTCA
GAAAAGATACCGTGCTGAATCAGTACCAGCTTTTCAGCATTGATATCCCGGATGACCAGATAGATTACCTGGATAAAGCG
TGGCTCCCCGAGGACTGGCAGGAAAATCCGGCGCCGGTATCGACAATGGATCTGGGAACCGGTTGGTTGCAGGCTAACAG
TGCCCTCGCGCTTATCTTACCTTCCTGTATCATTCCTTATGAAAACAATGCCATCCTTAACCCCTTACATCCGGCGTTCC
ACTCGGCGCTCAATTCAGTTCAGCAACTCCCCTTTATTTTTGATACCCGCCTGGCTGAAAAAACCGCACCCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16294.64 Da
• Isoelectric Point: 8.6042

>AT66187 WP_069598066.1 NZ_CP017184:c1530827-1530384 [Enterobacter roggenkampii]
MRTWIPAQKPADNALWRYAGLPANRGMRLIEFLNQGLPVSVLDNIHEWTSMSKADILRVTGINERNVARRKSAGRTLTPG
ESERVARFVRVLDAAVDYFGSKEEAWTWLQAPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT66187 NZ_CP017184:c1530827-1530384 [Enterobacter roggenkampii]
ATGAGAACATGGATCCCCGCACAGAAACCTGCTGATAACGCACTTTGGCGTTACGCCGGTTTACCCGCCAACCGAGGTAT
GCGGTTGATTGAATTTCTCAATCAGGGATTACCCGTCAGTGTGCTCGACAACATCCACGAGTGGACGTCCATGTCGAAAG
CGGACATTTTGCGTGTCACGGGTATCAACGAACGTAACGTGGCCCGCCGCAAAAGCGCGGGACGGACGTTAACGCCCGGC
GAGAGCGAACGCGTGGCCCGTTTCGTTCGGGTGCTGGATGCTGCCGTTGACTATTTTGGCAGTAAAGAAGAAGCATGGAC
CTGGCTGCAGGCACCGGTGCGCGGACTGGGGAACGTGGCGCCCGTCGACTTAATAGCGACGGAAACCGGTGCGCTGGAAG
TCACCGATTTGATTGGTCGCCTGGAACACGGCGTGTTCGCATGA

Structures

Toxin

• AlphaFold DB: A0A8B2SFJ4