Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 597889..598465 Replicon chromosome
Accession NZ_CP017184
Organism Enterobacter roggenkampii strain DSM 16690

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag BFV67_RS02745 Protein ID WP_025912099.1
Coordinates 597889..598176 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7D6YIG8
Locus tag BFV67_RS02750 Protein ID WP_025912101.1
Coordinates 598163..598465 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV67_RS23115 592902..593018 + 117 Protein_522 amino acid-binding protein -
BFV67_RS02720 593011..593706 + 696 WP_069597840.1 winged helix-turn-helix domain-containing protein -
BFV67_RS02725 593852..594322 + 471 WP_008501325.1 MarR family transcriptional regulator -
BFV67_RS02730 594319..595386 + 1068 WP_088728436.1 HlyD family secretion protein -
BFV67_RS02735 595376..596452 + 1077 WP_069597841.1 DUF2955 domain-containing protein -
BFV67_RS02740 596449..597720 - 1272 WP_069597842.1 DUF445 domain-containing protein -
BFV67_RS02745 597889..598176 + 288 WP_025912099.1 BrnT family toxin Toxin
BFV67_RS02750 598163..598465 + 303 WP_025912101.1 BrnA antitoxin family protein Antitoxin
BFV67_RS02755 598496..599134 - 639 WP_069597843.1 LysE family translocator -
BFV67_RS02760 599182..599925 - 744 WP_069597844.1 AraC family transcriptional regulator -
BFV67_RS02765 600077..601447 + 1371 WP_069597845.1 NAD-dependent succinate-semialdehyde dehydrogenase -
BFV67_RS02770 601493..601813 - 321 WP_008501314.1 hypothetical protein -
BFV67_RS02775 601813..602358 - 546 WP_008501313.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T66185 WP_025912099.1 NZ_CP017184:597889-598176 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T66185 NZ_CP017184:597889-598176 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTTGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGTCTTCTTGTTATTCTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11413.02 Da        Isoelectric Point: 10.0592

>AT66185 WP_025912101.1 NZ_CP017184:598163-598465 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARREAELKALAEKPEAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT66185 NZ_CP017184:598163-598465 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGAGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGAAGCAGAGATTGATTACAGCGATATCCCTGCTACGGACGATGAACAGTGGTCAGGTGCGGTGCGAGGAAAGT
TTTATCGTCCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A7D6YIG8

References