Detailed information of TA system TA066184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 260884..261481
• Replicon: chromosome
• Accession: NZ_CP017184
• Organism: Enterobacter roggenkampii strain DSM 16690

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BFV67_RS01165
• Protein ID: WP_069597753.1
• Coordinates: 261164..261481 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: W7P674
• Locus tag: BFV67_RS01160
• Protein ID: WP_021242718.1
• Coordinates: 260884..261171 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV67_RS01155	259256..260887	+	1632	WP_008503411.1	Na/Pi cotransporter family protein	-
BFV67_RS01160	260884..261171	-	288	WP_021242718.1	putative addiction module antidote protein	Antitoxin
BFV67_RS01165	261164..261481	-	318	WP_069597753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFV67_RS01170	261679..262551	+	873	WP_008503414.1	23S rRNA pseudouridine(2604) synthase RluF	-
BFV67_RS01175	262552..262824	-	273	WP_014830263.1	DUF3811 domain-containing protein	-
BFV67_RS01180	262875..263819	-	945	WP_023293551.1	ketopantoate/pantoate/pantothenate transporter PanS	-
BFV67_RS01185	263925..265499	-	1575	WP_069597754.1	RNA repair transcriptional activator RtcR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-91)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11835.67 Da
• Isoelectric Point: 9.6036

>T66184 WP_069597753.1 NZ_CP017184:c261481-261164 [Enterobacter roggenkampii]
MKEIVQTESFRRWEQNLKARRAKTIIASRLFRLANGLAGDVKPVGEGISELRIHFGPGYRIYFKDQGNYIIVLLCGGDKS
SQARDILMEKNAEQCIPMAGVNEHA

Nucleotide

• Length: 318 bp

>T66184 NZ_CP017184:c261481-261164 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGCTCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGAAAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGAATGAGCATGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10156.57 Da
• Isoelectric Point: 6.4596

>AT66184 WP_021242718.1 NZ_CP017184:c261171-260884 [Enterobacter roggenkampii]
MHKLTPYDPANALVDDEEIAVFIADALETGDSTYIAKALGVVARAKGMSNISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGFGLTLKHAGD

Nucleotide

• Length: 288 bp

>AT66184 NZ_CP017184:c261171-260884 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: W7P674