Detailed information of TA system TA066183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 52420..53081
• Replicon: chromosome
• Accession: NZ_CP017184
• Organism: Enterobacter roggenkampii strain DSM 16690

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BFV67_RS00255
• Protein ID: WP_023293716.1
• Coordinates: 52683..53081 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A837LFT0
• Locus tag: BFV67_RS00250
• Protein ID: WP_047748111.1
• Coordinates: 52420..52686 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV67_RS00225	48474..49802	-	1329	WP_023326395.1	PTS sugar transporter subunit IIC	-
BFV67_RS00230	49814..50128	-	315	WP_008500132.1	PTS sugar transporter subunit IIB	-
BFV67_RS00235	50422..51372	+	951	WP_069597711.1	LacI family transcriptional regulator	-
BFV67_RS00240	51434..51730	-	297	WP_059365122.1	hypothetical protein	-
BFV67_RS00245	51871..52290	+	420	WP_069597712.1	GNAT family N-acetyltransferase	-
BFV67_RS00250	52420..52686	+	267	WP_047748111.1	virulence protein	Antitoxin
BFV67_RS00255	52683..53081	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
BFV67_RS00260	53221..54021	+	801	WP_023293717.1	lipoprotein NlpA	-
BFV67_RS00265	54145..54924	+	780	WP_069597713.1	MBL fold metallo-hydrolase	-
BFV67_RS00270	54951..55904	+	954	WP_069597714.1	helix-turn-helix domain-containing protein	-
BFV67_RS00275	56144..57784	+	1641	WP_069598930.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T66183 WP_023293716.1 NZ_CP017184:52683-53081 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T66183 NZ_CP017184:52683-53081 [Enterobacter roggenkampii]
ATGAGACATATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCT
GGCGCCGGGTGACGTTTGTATCTCAAGCATAACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGAT
TGAAAGAAACAATCCGGGAGTTTCTCAATACCATCACGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAA
CTCCGTGCAACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGG
AACAACGATCGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9950.31 Da
• Isoelectric Point: 4.8145

>AT66183 WP_047748111.1 NZ_CP017184:52420-52686 [Enterobacter roggenkampii]
MERTAKLFKKGQNQAVMLPAEFAFNAESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT66183 NZ_CP017184:52420-52686 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAAAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTAATGCGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A837LFT0