Detailed information of TA system TA066170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1093605..1094225
• Replicon: chromosome
• Accession: NZ_CP017183
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain LMG27195

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A837FFM2
• Locus tag: BFV63_RS05115
• Protein ID: WP_015571250.1
• Coordinates: 1093605..1093823 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: BFV63_RS05120
• Protein ID: WP_048241282.1
• Coordinates: 1093851..1094225 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV63_RS05085	1089617..1089877	+	261	WP_015571255.1	type B 50S ribosomal protein L31	-
BFV63_RS05090	1089880..1090020	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
BFV63_RS05095	1090017..1090727	-	711	WP_048241285.1	GNAT family N-acetyltransferase	-
BFV63_RS05100	1090829..1092289	+	1461	WP_032608667.1	PLP-dependent aminotransferase family protein	-
BFV63_RS05105	1092261..1092728	-	468	WP_022650495.1	hypothetical protein	-
BFV63_RS05110	1092845..1093396	-	552	WP_048241283.1	maltose O-acetyltransferase	-
BFV63_RS05115	1093605..1093823	-	219	WP_015571250.1	hemolysin expression modulator Hha	Toxin
BFV63_RS05120	1093851..1094225	-	375	WP_048241282.1	Hha toxicity modulator TomB	Antitoxin
BFV63_RS05125	1094736..1097882	-	3147	WP_022650497.1	multidrug efflux RND transporter permease subunit	-
BFV63_RS05130	1097905..1099098	-	1194	WP_022650498.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T66170 WP_015571250.1 NZ_CP017183:c1093823-1093605 [Enterobacter hormaechei subsp. xiangfangensis]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T66170 NZ_CP017183:c1093823-1093605 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14470.25 Da
• Isoelectric Point: 4.8989

>AT66170 WP_048241282.1 NZ_CP017183:c1094225-1093851 [Enterobacter hormaechei subsp. xiangfangensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALSYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT66170 NZ_CP017183:c1094225-1093851 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAGTTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A837FFM2