Detailed information of TA system TA066166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 3596509..3597536
• Replicon: chromosome
• Accession: NZ_CP017181
• Organism: Enterobacter kobei strain DSM 13645

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: BFV64_RS17275
• Protein ID: WP_014884911.1
• Coordinates: 3596509..3597096 (-)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: -
• Locus tag: BFV64_RS17280
• Protein ID: WP_080021220.1
• Coordinates: 3597138..3597536 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV64_RS17255	3591859..3593622	+	1764	WP_069602310.1	terminase ATPase subunit family protein	-
BFV64_RS17260	3593622..3594674	+	1053	WP_069602311.1	phage portal protein	-
BFV64_RS17265	3594706..3596145	-	1440	WP_069602312.1	TIR domain-containing protein	-
BFV64_RS17275	3596509..3597096	-	588	WP_014884911.1	hypothetical protein	Toxin
BFV64_RS17280	3597138..3597536	-	399	WP_080021220.1	DUF4065 domain-containing protein	Antitoxin
BFV64_RS17285	3598017..3598250	-	234	WP_014884913.1	DinI family protein	-
BFV64_RS17290	3598261..3598449	-	189	WP_014884914.1	hypothetical protein	-
BFV64_RS17295	3598609..3599304	-	696	WP_069602314.1	DNA adenine methylase	-
BFV64_RS17300	3599456..3601759	-	2304	WP_069602315.1	replication endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3585187..3606178	20991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin SocA-like, Panacea domain (4-100)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21839.54 Da
• Isoelectric Point: 4.5519

>T66166 WP_014884911.1 NZ_CP017181:c3597096-3596509 [Enterobacter kobei]
MSIDNDLFESILRGAISANIDDLVKTITATEPQQPSKDSPQEEDDDVGDDKAQKEAQAFDTPTDDEHEELQALGYWSLSH
QRQVDSLTIQKSKEEIVRISCENDELRAKTKNNHAMARGRRIDNRLRLQMAAATFRFMQYWCCFVALVVFIYVAKNDGNP
PSEVIIALLGTSTISIVGLVGFVVSGLFKSNKDKE

Nucleotide

• Length: 588 bp

>T66166 NZ_CP017181:c3597096-3596509 [Enterobacter kobei]
ATGTCCATTGATAACGATCTGTTCGAAAGCATACTTAGAGGCGCTATTAGCGCTAATATTGACGATCTTGTTAAAACTAT
CACTGCCACTGAGCCTCAACAGCCTTCTAAAGATTCCCCGCAAGAAGAAGATGATGATGTGGGAGATGATAAAGCTCAAA
AAGAGGCGCAAGCCTTTGATACTCCTACTGATGATGAGCATGAGGAACTTCAAGCATTAGGCTATTGGAGTTTGTCGCAT
CAAAGACAAGTTGATTCCTTGACTATACAAAAGTCTAAAGAAGAAATTGTTCGGATTAGTTGCGAAAATGACGAGTTGCG
AGCAAAAACAAAGAATAACCATGCTATGGCTAGAGGGCGACGTATCGATAATCGGTTACGTTTGCAGATGGCTGCTGCTA
CGTTCCGTTTCATGCAATACTGGTGTTGCTTTGTTGCATTAGTGGTCTTCATTTACGTAGCAAAAAATGACGGTAATCCC
CCCAGTGAAGTAATAATTGCTCTCCTTGGAACAAGCACGATTAGCATTGTTGGTTTGGTTGGTTTTGTGGTCAGTGGATT
ATTTAAAAGTAATAAAGATAAAGAGTAG

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14970.23 Da
• Isoelectric Point: 6.7100

>AT66166 WP_080021220.1 NZ_CP017181:c3597536-3597138 [Enterobacter kobei]
MQAQKLLYIAHGYSLAFLNEPLLEEPVCAWRYGPVIPSVYYALRHNRSEPIADKAPTTAIETETLDSKTASLLDMVYETY
GKLSGVVLSEFTHRPGTPWYQAMVKRENIISDASIKDYYLKLIKRDPVCNGL

Nucleotide

• Length: 399 bp

>AT66166 NZ_CP017181:c3597536-3597138 [Enterobacter kobei]
ATGCAGGCCCAGAAACTGCTGTACATAGCTCATGGCTATAGCTTAGCGTTTCTAAATGAGCCTTTGCTTGAAGAACCCGT
GTGTGCGTGGAGGTATGGTCCGGTCATACCGTCCGTGTATTATGCTCTGCGTCACAATCGTTCTGAACCGATTGCAGATA
AGGCACCGACAACTGCAATTGAAACAGAAACCTTAGATAGCAAAACAGCTTCTTTGCTTGATATGGTTTACGAAACTTAT
GGCAAGCTTTCTGGAGTTGTTCTCTCTGAGTTTACACACCGTCCGGGTACACCTTGGTATCAAGCCATGGTCAAGAGAGA
GAATATAATCAGTGACGCAAGTATCAAAGATTACTATCTAAAACTGATAAAGCGTGACCCGGTTTGCAACGGATTGTAA