Detailed information of TA system TA066165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2991166..2991756
• Replicon: chromosome
• Accession: NZ_CP017181
• Organism: Enterobacter kobei strain DSM 13645

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8B2Y1G2
• Locus tag: BFV64_RS14440
• Protein ID: WP_045134957.1
• Coordinates: 2991166..2991498 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BFV64_RS14445
• Protein ID: WP_045134956.1
• Coordinates: 2991499..2991756 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV64_RS14425	2987330..2988655	+	1326	WP_045281559.1	SidA/IucD/PvdA family monooxygenase	-
BFV64_RS14430	2988682..2990871	+	2190	WP_063614372.1	TonB-dependent siderophore receptor	-
BFV64_RS14440	2991166..2991498	-	333	WP_045134957.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BFV64_RS14445	2991499..2991756	-	258	WP_045134956.1	antitoxin	Antitoxin
BFV64_RS14450	2992104..2992310	-	207	WP_045134955.1	helix-turn-helix domain-containing protein	-
BFV64_RS14455	2992307..2992774	-	468	Protein_2848	hypothetical protein	-
BFV64_RS14460	2993067..2996375	+	3309	Protein_2849	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11785.63 Da
• Isoelectric Point: 10.5834

>T66165 WP_045134957.1 NZ_CP017181:c2991498-2991166 [Enterobacter kobei]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLDGAGTKTMGAIRCDQPRTI
DMAARSGKRLERIPDAVVNEVLARLDAILN

Nucleotide

• Length: 333 bp

>T66165 NZ_CP017181:c2991498-2991166 [Enterobacter kobei]
ATGGACAGAGGGGAAATTTGGCTCGTCTCACTGGATCCTACAGCTGGACATGAACAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCTTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAACTTTGCACGTA
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACGATGGGCGCTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAGCGGCAAGCGGCTGGAACGCATACCAGACGCTGTGGTGAATGAGGTGCTTGCCAGGCTGGATGC
GATTCTGAATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9311.83 Da
• Isoelectric Point: 4.7512

>AT66165 WP_045134956.1 NZ_CP017181:c2991756-2991499 [Enterobacter kobei]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTINNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEGREWIDAPVV
GKEIL

Nucleotide

• Length: 258 bp

>AT66165 NZ_CP017181:c2991756-2991499 [Enterobacter kobei]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTTGGGTT
GTCAACGGACAGCGAAGTCGGCATGACCATAAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTTGCACAGTGCGATCCGCACGCCGAAATGAGCGACGAGGGTCGGGAATGGATTGATGCGCCTGTGGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B2Y1G2