Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1084419..1085039 Replicon chromosome
Accession NZ_CP017181
Organism Enterobacter kobei strain DSM 13645

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag BFV64_RS05135 Protein ID WP_014882802.1
Coordinates 1084419..1084637 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag BFV64_RS05140 Protein ID WP_008499288.1
Coordinates 1084665..1085039 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV64_RS05105 1080428..1080688 + 261 WP_069602468.1 type B 50S ribosomal protein L31 -
BFV64_RS05110 1080691..1080831 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BFV64_RS05115 1080828..1081538 - 711 WP_069601783.1 GNAT family N-acetyltransferase -
BFV64_RS05120 1081640..1083100 + 1461 WP_014882799.1 PLP-dependent aminotransferase family protein -
BFV64_RS05125 1083072..1083539 - 468 WP_014882800.1 membrane protein -
BFV64_RS05130 1083658..1084209 - 552 WP_014882801.1 maltose O-acetyltransferase -
BFV64_RS05135 1084419..1084637 - 219 WP_014882802.1 hemolysin expression modulator Hha Toxin
BFV64_RS05140 1084665..1085039 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
BFV64_RS05145 1085551..1088697 - 3147 WP_069601784.1 multidrug efflux RND transporter permease subunit AcrB -
BFV64_RS05150 1088720..1089913 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T66155 WP_014882802.1 NZ_CP017181:c1084637-1084419 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T66155 NZ_CP017181:c1084637-1084419 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGCCGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATTCCCACATCCGTGTGGAAATTTGTACGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT66155 WP_008499288.1 NZ_CP017181:c1085039-1084665 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT66155 NZ_CP017181:c1085039-1084665 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCGCAAGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References