Detailed information of TA system TA066152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4634323..4634939
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BFV66_RS22300
• Protein ID: WP_080480776.1
• Coordinates: 4634323..4634694 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: BFV66_RS22305
• Protein ID: WP_015569912.1
• Coordinates: 4634697..4634939 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS22285	4631823..4632725	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
BFV66_RS22290	4632722..4633357	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
BFV66_RS22295	4633354..4634283	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
BFV66_RS22300	4634323..4634694	-	372	WP_080480776.1	type II toxin-antitoxin system VapC family toxin	Toxin
BFV66_RS22305	4634697..4634939	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
BFV66_RS22310	4635138..4636058	+	921	WP_047051644.1	alpha/beta hydrolase	-
BFV66_RS22315	4636067..4637008	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
BFV66_RS22320	4637053..4637490	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BFV66_RS22325	4637487..4638368	-	882	WP_003861949.1	virulence factor BrkB family protein	-
BFV66_RS22330	4638362..4638961	-	600	WP_032649898.1	glucose-1-phosphatase	-
BFV66_RS22335	4639080..4639880	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13792.96 Da
• Isoelectric Point: 6.7561

>T66152 WP_080480776.1 NZ_CP017180:c4634694-4634323 [Enterobacter hormaechei subsp. oharae]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQRRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T66152 NZ_CP017180:c4634694-4634323 [Enterobacter hormaechei subsp. oharae]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGAAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT66152 WP_015569912.1 NZ_CP017180:c4634939-4634697 [Enterobacter hormaechei subsp. oharae]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT66152 NZ_CP017180:c4634939-4634697 [Enterobacter hormaechei subsp. oharae]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3