Detailed information of TA system TA066150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3114150..3114889
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: BFV66_RS14890
• Protein ID: WP_045329830.1
• Coordinates: 3114404..3114889 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: BFV66_RS14885
• Protein ID: WP_047050951.1
• Coordinates: 3114150..3114416 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS14865	3109626..3110612	+	987	WP_047050955.1	DUF979 domain-containing protein	-
BFV66_RS14870	3110622..3111617	+	996	WP_047050954.1	DUF2891 domain-containing protein	-
BFV66_RS14875	3111639..3113027	-	1389	WP_047050952.1	MFS transporter	-
BFV66_RS14880	3113157..3114086	+	930	WP_047050986.1	LysR family transcriptional regulator	-
BFV66_RS14885	3114150..3114416	+	267	WP_047050951.1	DUF1778 domain-containing protein	Antitoxin
BFV66_RS14890	3114404..3114889	+	486	WP_045329830.1	GNAT family N-acetyltransferase	Toxin
BFV66_RS14895	3115067..3116359	-	1293	WP_047050950.1	glycoside hydrolase	-
BFV66_RS14900	3116464..3117222	-	759	WP_015570520.1	DeoR/GlpR family DNA-binding transcription regulator	-
BFV66_RS14905	3117309..3117893	+	585	WP_047050949.1	NUDIX domain-containing protein	-
BFV66_RS14910	3117978..3118736	+	759	WP_047050948.1	trans-aconitate 2-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17512.18 Da
• Isoelectric Point: 9.9658

>T66150 WP_045329830.1 NZ_CP017180:3114404-3114889 [Enterobacter hormaechei subsp. oharae]
VGRVTAPEPLSSAHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T66150 NZ_CP017180:3114404-3114889 [Enterobacter hormaechei subsp. oharae]
GTGGGACGTGTAACAGCACCAGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTAAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10089.51 Da
• Isoelectric Point: 4.4857

>AT66150 WP_047050951.1 NZ_CP017180:3114150-3114416 [Enterobacter hormaechei subsp. oharae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVANDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT66150 NZ_CP017180:3114150-3114416 [Enterobacter hormaechei subsp. oharae]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTATGCCGAGTTCATTGACATGCTCGATGCCCCGGTCGCGAACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA