Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1702357..1703154 Replicon chromosome
Accession NZ_CP017180
Organism Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag BFV66_RS08115 Protein ID WP_032670168.1
Coordinates 1702633..1703154 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag BFV66_RS08110 Protein ID WP_001303459.1
Coordinates 1702357..1702626 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV66_RS08080 1697897..1698256 + 360 WP_040117469.1 helix-turn-helix transcriptional regulator -
BFV66_RS08085 1698582..1699823 + 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
BFV66_RS08090 1699856..1700083 - 228 WP_006809324.1 YccJ family protein -
BFV66_RS08095 1700102..1700698 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
BFV66_RS08100 1701090..1701260 + 171 WP_001273664.1 general stress protein -
BFV66_RS08105 1701377..1702282 + 906 WP_047051684.1 DMT family transporter -
BFV66_RS08110 1702357..1702626 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
BFV66_RS08115 1702633..1703154 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
BFV66_RS08120 1703213..1704535 - 1323 WP_048982825.1 pyrimidine utilization transport protein G -
BFV66_RS08125 1704557..1705048 - 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
BFV66_RS08130 1705061..1705651 - 591 WP_047052687.1 malonic semialdehyde reductase -
BFV66_RS08135 1705661..1706461 - 801 WP_047052686.1 pyrimidine utilization protein D -
BFV66_RS08140 1706469..1706855 - 387 WP_032670161.1 pyrimidine utilization protein C -
BFV66_RS08145 1706867..1707556 - 690 WP_047052685.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T66141 WP_032670168.1 NZ_CP017180:1702633-1703154 [Enterobacter hormaechei subsp. oharae]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T66141 NZ_CP017180:1702633-1703154 [Enterobacter hormaechei subsp. oharae]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGGTACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT66141 WP_001303459.1 NZ_CP017180:1702357-1702626 [Enterobacter hormaechei subsp. oharae]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT66141 NZ_CP017180:1702357-1702626 [Enterobacter hormaechei subsp. oharae]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References