Detailed information of TA system TA066138

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 949377..950086
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7D6YAQ1
• Locus tag: BFV66_RS22855
• Protein ID: WP_071886833.1
• Coordinates: 949718..950086 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: BFV66_RS04515
• Protein ID: WP_047051179.1
• Coordinates: 949377..949697 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS04475	944937..945771	+	835	Protein_863	DUF945 domain-containing protein	-
BFV66_RS04480	945973..946677	+	705	WP_047051185.1	WYL domain-containing protein	-
BFV66_RS04485	946674..947288	+	615	WP_047051184.1	hypothetical protein	-
BFV66_RS04490	947377..947787	+	411	WP_047051183.1	hypothetical protein	-
BFV66_RS04495	947864..948100	+	237	WP_047051182.1	DUF905 domain-containing protein	-
BFV66_RS04500	948180..948638	+	459	WP_047051181.1	antirestriction protein	-
BFV66_RS04505	948647..949129	+	483	WP_075211782.1	DNA repair protein RadC	-
BFV66_RS04510	949138..949359	+	222	WP_016243789.1	DUF987 domain-containing protein	-
BFV66_RS04515	949377..949697	+	321	WP_047051179.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFV66_RS22855	949718..950086	+	369	WP_071886833.1	TA system toxin CbtA family protein	Toxin
BFV66_RS04520	950432..951178	-	747	WP_047051178.1	class I SAM-dependent methyltransferase	-
BFV66_RS04525	951189..951446	-	258	WP_003863178.1	YjhX family toxin	-
BFV66_RS04530	951706..953643	-	1938	WP_047051177.1	bifunctional diguanylate cyclase/phosphodiesterase	-
BFV66_RS04535	953661..954155	-	495	WP_047051176.1	oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	938675..950086	11411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (6-105)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13738.90 Da
• Isoelectric Point: 8.1347

>T66138 WP_071886833.1 NZ_CP017180:949718-950086 [Enterobacter hormaechei subsp. oharae]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDQTVIQEHIDAGITLANAVNFLVEKYELVRIDRTGF
TSQEQAPYLTVTDILHARRACGLMNSCSYREVSNIVLSRSRQ

Nucleotide

• Length: 369 bp

>T66138 NZ_CP017180:949718-950086 [Enterobacter hormaechei subsp. oharae]
ATGAAAACTTTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCTCCAGTGGCTGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATCAAACCGTTATACAGGAACATATCG
ATGCGGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAAAAGTATGAGTTAGTACGTATCGATCGCACAGGATTT
ACCTCACAGGAACAAGCGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTG
TTCGTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGCAGTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11901.60 Da
• Isoelectric Point: 7.2633

>AT66138 WP_047051179.1 NZ_CP017180:949377-949697 [Enterobacter hormaechei subsp. oharae]
MRSNATWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPHFISLMESMLTTGEMNPRHAHCVTLY
RNGFTCEADTLGSCGYLYIVVYPTQS

Nucleotide

• Length: 321 bp

>AT66138 NZ_CP017180:949377-949697 [Enterobacter hormaechei subsp. oharae]
ATGAGGAGTAACGCTACATGGGGACTGCAACGTGATATCACACCGCGTCTGGGAGCCCGTCTGGTGCAGGAGGGCAACCG
GCTCCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAATTCAGTGACGCCGAATGCCTGAAGTTGGATGTCGCATTCC
CGCATTTTATCAGTCTGATGGAATCAATGCTGACTACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTAC
CGCAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGCTGCGGCTACTTATATATCGTTGTTTATCCCACTCAAAGCTA
A

Structures

Toxin

• AlphaFold DB: A0A7D6YAQ1