Detailed information of TA system TA066136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 89182..89838
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BFV66_RS00455
• Protein ID: WP_080344062.1
• Coordinates: 89182..89574 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BFV66_RS00460
• Protein ID: WP_047050676.1
• Coordinates: 89578..89838 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS00430	84842..85477	-	636	WP_047050682.1	LuxR family transcriptional regulator	-
BFV66_RS00435	86048..86668	+	621	WP_047050680.1	response regulator transcription factor	-
BFV66_RS00440	86744..87424	+	681	WP_047050679.1	EAL domain-containing protein	-
BFV66_RS00445	87458..88258	-	801	WP_047050678.1	lipoprotein NlpA	-
BFV66_RS00450	88640..89113	+	474	WP_032674176.1	hypothetical protein	-
BFV66_RS00455	89182..89574	-	393	WP_080344062.1	type II toxin-antitoxin system VapC family toxin	Toxin
BFV66_RS00460	89578..89838	-	261	WP_047050676.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BFV66_RS00465	89956..91455	-	1500	WP_047050674.1	MFS transporter	-
BFV66_RS00470	91534..92181	+	648	WP_017383663.1	TetR/AcrR family transcriptional regulator	-
BFV66_RS00475	92178..92597	-	420	WP_047050673.1	GNAT family N-acetyltransferase	-
BFV66_RS00480	92783..93352	+	570	WP_032103771.1	TetR/AcrR family transcriptional regulator	-
BFV66_RS00485	93500..94621	+	1122	WP_047050671.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	78731..89838	11107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14585.78 Da
• Isoelectric Point: 5.8985

>T66136 WP_080344062.1 NZ_CP017180:c89574-89182 [Enterobacter hormaechei subsp. oharae]
MLHMLDTNIVSHLVRQHPEVVNRYSQITPEKMCISSVTEAELLYGVAKKQNNKLHETIMEFLKTITVCAWDSEAAAIYGE
LRAAMEKKGKVMGDLDQLIAAHAISRGTTIVTNDHAFGMVQDLTVEDWTQ

Nucleotide

• Length: 393 bp

>T66136 NZ_CP017180:c89574-89182 [Enterobacter hormaechei subsp. oharae]
ATGCTGCATATGCTGGACACTAACATCGTAAGCCATCTGGTGAGGCAGCATCCTGAAGTTGTAAACCGATATTCGCAAAT
TACGCCTGAAAAGATGTGTATTTCAAGCGTAACGGAAGCGGAATTGCTTTACGGCGTAGCCAAAAAGCAAAACAACAAGC
TGCACGAAACCATAATGGAATTTCTTAAAACCATCACTGTCTGCGCCTGGGATAGCGAAGCCGCAGCCATCTACGGTGAA
CTGCGTGCGGCAATGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAGTTGATTGCCGCACATGCTATCAGCCGGGG
CACGACGATTGTCACGAACGATCATGCGTTCGGGATGGTACAGGATCTTACGGTTGAGGACTGGACTCAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10084.38 Da
• Isoelectric Point: 9.6899

>AT66136 WP_047050676.1 NZ_CP017180:c89838-89578 [Enterobacter hormaechei subsp. oharae]
MERTAKLFKKGRNQAVVLPAEFAFETESVYIRRDDEGNVVLTARSEKARHRDNFLRMLKETHVPDTFLSKEERNQSYTTR
NPLEGL

Nucleotide

• Length: 261 bp

>AT66136 NZ_CP017180:c89838-89578 [Enterobacter hormaechei subsp. oharae]
ATGGAACGCACAGCGAAACTATTCAAAAAAGGACGAAACCAGGCCGTGGTCTTGCCTGCGGAATTTGCGTTTGAAACGGA
GAGTGTTTATATCCGCCGGGATGACGAGGGGAATGTGGTTTTGACGGCAAGGTCCGAGAAAGCACGGCACAGGGATAACT
TTTTGCGCATGTTAAAAGAAACGCATGTGCCTGATACCTTTCTAAGTAAAGAGGAGCGCAATCAGAGCTATACGACAAGA
AACCCTCTTGAAGGGCTGTAG