Detailed information of TA system TA066133

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 48083..48750
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: BFV66_RS00245
• Protein ID: WP_059443702.1
• Coordinates: 48430..48750 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: BFV66_RS00240
• Protein ID: WP_059443453.1
• Coordinates: 48083..48409 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS00210	43282..44661	-	1380	WP_015570106.1	carbohydrate porin	-
BFV66_RS00215	44798..45100	-	303	WP_014168006.1	PTS lactose/cellobiose transporter subunit IIA	-
BFV66_RS23685	45106..45306	-	201	Protein_45	glycoside hydrolase family 1 protein	-
BFV66_RS00225	45379..46499	+	1121	WP_085950818.1	IS3-like element ISSen4 family transposase	-
BFV66_RS00230	46710..47531	+	822	WP_015703839.1	DUF945 domain-containing protein	-
BFV66_RS00235	47600..48070	+	471	WP_015703840.1	DNA repair protein RadC	-
BFV66_RS00240	48083..48409	+	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFV66_RS00245	48430..48750	+	321	WP_059443702.1	TA system toxin CbtA family protein	Toxin
BFV66_RS00250	49045..50601	+	1557	WP_047050722.1	hypothetical protein	-
BFV66_RS22740	50641..50817	-	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
BFV66_RS00255	50954..51295	+	342	WP_006812446.1	SymE family type I addiction module toxin	-
BFV66_RS23495	51398..51650	+	253	Protein_54	DNA-binding protein	-
BFV66_RS00260	51923..52273	+	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BFV66_RS00265	52270..52569	+	300	WP_045335986.1	XRE family transcriptional regulator	-
BFV66_RS00270	52634..53515	-	882	WP_047050719.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	45379..66936	21557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-99)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12048.92 Da
• Isoelectric Point: 6.2120

>T66133 WP_059443702.1 NZ_CP017180:48430-48750 [Enterobacter hormaechei subsp. oharae]
MNTQPATTPQAAKPCLSPLAVWKMLLTILLDQHYGLTLNDTPFSDETVIQKHIEAGITLADAINFLVERYELVRIDQKGF
SVQDQEPWLTSMDVHRARFKLNVARL

Nucleotide

• Length: 321 bp

>T66133 NZ_CP017180:48430-48750 [Enterobacter hormaechei subsp. oharae]
ATGAATACTCAACCTGCAACAACTCCGCAGGCGGCGAAGCCATGTCTGTCGCCCCTGGCTGTCTGGAAAATGTTACTGAC
AATCCTGCTAGACCAGCACTATGGTCTCACGCTAAACGACACGCCGTTCAGTGATGAAACTGTTATTCAGAAACACATTG
AGGCAGGTATTACCTTGGCTGATGCCATAAATTTTCTGGTTGAACGCTATGAACTAGTTCGTATTGACCAAAAAGGCTTC
TCTGTGCAAGATCAAGAACCTTGGTTAACTTCTATGGATGTCCACCGAGCACGATTTAAACTCAACGTGGCACGTTTATA
A

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12277.99 Da
• Isoelectric Point: 6.3620

>AT66133 WP_059443453.1 NZ_CP017180:48083-48409 [Enterobacter hormaechei subsp. oharae]
MSLIPAHEWGLQREIVPRFGARLVQEGNRLHYLADRASLMGNFSDTECFKLNDAFPHFISQMESMLTTGELIPCHHHCVT
LYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 327 bp

>AT66133 NZ_CP017180:48083-48409 [Enterobacter hormaechei subsp. oharae]
ATGTCATTAATACCAGCCCATGAATGGGGACTACAAAGAGAAATTGTTCCACGGTTTGGTGCCAGATTAGTTCAGGAAGG
CAACCGGCTGCATTATCTGGCTGACAGGGCTAGTTTGATGGGCAATTTCAGCGATACGGAATGTTTCAAATTGAATGACG
CATTTCCACACTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAACTGATCCCCTGTCACCATCACTGTGTCACT
CTCTACCACAACGGTTTTACCTGCGAAGCTGACACCCTTGGCAGTTGCGGCTACGTGTACATCGCTGTTTACCCCACTCA
ACGCTAA