Detailed information of TA system TA066132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4694385..4695001
• Replicon: chromosome
• Accession: NZ_CP017179
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain DSM 16691

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BFV68_RS22285
• Protein ID: WP_077266491.1
• Coordinates: 4694385..4694756 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: BFV68_RS22290
• Protein ID: WP_015569912.1
• Coordinates: 4694759..4695001 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV68_RS22270	4691885..4692787	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
BFV68_RS22275	4692784..4693419	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
BFV68_RS22280	4693416..4694345	+	930	WP_006808712.1	formate dehydrogenase accessory protein FdhE	-
BFV68_RS22285	4694385..4694756	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
BFV68_RS22290	4694759..4695001	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
BFV68_RS22295	4695200..4696120	+	921	WP_058687689.1	alpha/beta hydrolase	-
BFV68_RS22300	4696129..4697070	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
BFV68_RS22305	4697115..4697552	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BFV68_RS22310	4697549..4698430	-	882	WP_003861949.1	virulence factor BrkB family protein	-
BFV68_RS22315	4698424..4699023	-	600	WP_003861947.1	glucose-1-phosphatase	-
BFV68_RS22320	4699142..4699942	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T66132 WP_077266491.1 NZ_CP017179:c4694756-4694385 [Enterobacter hormaechei subsp. steigerwaltii]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T66132 NZ_CP017179:c4694756-4694385 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT66132 WP_015569912.1 NZ_CP017179:c4695001-4694759 [Enterobacter hormaechei subsp. steigerwaltii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT66132 NZ_CP017179:c4695001-4694759 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3