Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2298584..2299323 Replicon chromosome
Accession NZ_CP017179
Organism Enterobacter hormaechei subsp. steigerwaltii strain DSM 16691

Toxin (Protein)


Gene name tacT Uniprot ID A0A3L9PBP9
Locus tag BFV68_RS10865 Protein ID WP_003857133.1
Coordinates 2298584..2299069 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag BFV68_RS10870 Protein ID WP_003857131.1
Coordinates 2299057..2299323 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV68_RS10835 2294163..2294708 + 546 WP_032671141.1 NUDIX hydrolase -
BFV68_RS10840 2294892..2295527 - 636 WP_015570517.1 DUF421 domain-containing protein -
BFV68_RS10845 2295537..2295983 - 447 WP_032671143.1 DUF3290 domain-containing protein -
BFV68_RS10850 2296261..2296728 - 468 Protein_2120 hypothetical protein -
BFV68_RS10855 2296983..2297948 + 966 WP_033487773.1 hypothetical protein -
BFV68_RS10860 2297964..2298533 + 570 WP_033487774.1 hypothetical protein -
BFV68_RS10865 2298584..2299069 - 486 WP_003857133.1 GNAT family N-acetyltransferase Toxin
BFV68_RS10870 2299057..2299323 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
BFV68_RS10875 2299387..2300316 - 930 WP_069595824.1 LysR family transcriptional regulator -
BFV68_RS10880 2300446..2301828 + 1383 WP_047741273.1 MFS transporter -
BFV68_RS10885 2301850..2302845 - 996 WP_017382342.1 DUF2891 domain-containing protein -
BFV68_RS10890 2302855..2303841 - 987 WP_017382341.1 DUF979 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17540.23 Da        Isoelectric Point: 9.9658

>T66119 WP_003857133.1 NZ_CP017179:c2299069-2298584 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T66119 NZ_CP017179:c2299069-2298584 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCC
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT66119 WP_003857131.1 NZ_CP017179:c2299323-2299057 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT66119 NZ_CP017179:c2299323-2299057 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L9PBP9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References