Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1103149..1103769 Replicon chromosome
Accession NZ_CP017179
Organism Enterobacter hormaechei subsp. steigerwaltii strain DSM 16691

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag BFV68_RS05195 Protein ID WP_015571250.1
Coordinates 1103149..1103367 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag BFV68_RS05200 Protein ID WP_006809850.1
Coordinates 1103395..1103769 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV68_RS05165 1099161..1099421 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
BFV68_RS05170 1099424..1099564 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
BFV68_RS05175 1099561..1100271 - 711 WP_023303201.1 GNAT family N-acetyltransferase -
BFV68_RS05180 1100373..1101833 + 1461 WP_017694397.1 PLP-dependent aminotransferase family protein -
BFV68_RS05185 1101805..1102272 - 468 WP_017383209.1 membrane protein -
BFV68_RS05190 1102389..1102940 - 552 WP_017694396.1 maltose O-acetyltransferase -
BFV68_RS05195 1103149..1103367 - 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
BFV68_RS05200 1103395..1103769 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
BFV68_RS05205 1104280..1107426 - 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit -
BFV68_RS05210 1107449..1108642 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T66116 WP_015571250.1 NZ_CP017179:c1103367-1103149 [Enterobacter hormaechei subsp. steigerwaltii]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T66116 NZ_CP017179:c1103367-1103149 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT66116 WP_006809850.1 NZ_CP017179:c1103769-1103395 [Enterobacter hormaechei subsp. steigerwaltii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT66116 NZ_CP017179:c1103769-1103395 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References