Detailed information of TA system TA066115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 615425..616001
• Replicon: chromosome
• Accession: NZ_CP017179
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain DSM 16691

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: BFV68_RS02930
• Protein ID: WP_015572580.1
• Coordinates: 615425..615712 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: BFV68_RS02935
• Protein ID: WP_017694570.1
• Coordinates: 615699..616001 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV68_RS02905	610519..611237	+	719	Protein_559	winged helix-turn-helix transcriptional regulator	-
BFV68_RS02910	611387..611857	+	471	WP_033486706.1	MarR family transcriptional regulator	-
BFV68_RS02915	611854..612921	+	1068	WP_026080654.1	HlyD family secretion protein	-
BFV68_RS02920	612911..613987	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
BFV68_RS02925	613984..615255	-	1272	WP_033486707.1	DUF445 domain-containing protein	-
BFV68_RS02930	615425..615712	+	288	WP_015572580.1	BrnT family toxin	Toxin
BFV68_RS02935	615699..616001	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
BFV68_RS02940	616030..616668	-	639	WP_033486708.1	LysE family translocator	-
BFV68_RS02945	616707..617459	-	753	WP_033486709.1	AraC family transcriptional regulator	-
BFV68_RS02950	617613..618983	+	1371	WP_069595624.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
BFV68_RS02955	619159..619704	-	546	WP_033486712.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T66115 WP_015572580.1 NZ_CP017179:615425-615712 [Enterobacter hormaechei subsp. steigerwaltii]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T66115 NZ_CP017179:615425-615712 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCCTATAGAGTATGAGTGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT66115 WP_017694570.1 NZ_CP017179:615699-616001 [Enterobacter hormaechei subsp. steigerwaltii]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT66115 NZ_CP017179:615699-616001 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGCGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA