Detailed information of TA system TA066089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 175196..175963
• Replicon: chromosome
• Accession: NZ_CP017156
• Organism: Macrococcus sp. IME1552

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: BHM04_RS12615
• Protein ID: WP_165958672.1
• Coordinates: 175820..175963 (+)
• Length: 48 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: BHM04_RS00985
• Protein ID: WP_096075978.1
• Coordinates: 175196..175807 (+)
• Length: 204 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHM04_RS00960	170245..171651	+	1407	WP_096078059.1	ABC transporter permease subunit	-
BHM04_RS00965	171644..172366	+	723	WP_096075974.1	amino acid ABC transporter ATP-binding protein	-
BHM04_RS00970	172517..173641	+	1125	WP_096075975.1	tRNA epoxyqueuosine(34) reductase QueG	-
BHM04_RS00975	173642..174115	+	474	WP_096075976.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
BHM04_RS00980	174128..174865	+	738	WP_096075977.1	oxygen-insensitive NADPH nitroreductase	-
BHM04_RS00985	175196..175807	+	612	WP_096075978.1	glucosamine-6-phosphate isomerase	Antitoxin
BHM04_RS12615	175820..175963	+	144	WP_165958672.1	hypothetical protein	Toxin
BHM04_RS00990	176074..177459	+	1386	WP_096075979.1	class II fumarate hydratase	-
BHM04_RS00995	177490..179022	+	1533	WP_096075980.1	exopolyphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5413.64 Da
• Isoelectric Point: 3.6118

>T66089 WP_165958672.1 NZ_CP017156:175820-175963 [Macrococcus sp. IME1552]
MSTSHDNKNEMVTDFDDVVELGKEMEQISEQNAEAREEGQEQEDDPQ

Nucleotide

• Length: 144 bp

>T66089 NZ_CP017156:175820-175963 [Macrococcus sp. IME1552]
ATGTCAACATCACATGATAATAAAAATGAAATGGTAACTGATTTCGACGACGTTGTAGAATTAGGTAAAGAGATGGAGCA
AATCTCTGAACAAAATGCTGAAGCGCGAGAAGAAGGCCAGGAACAAGAGGATGATCCTCAATAA

Antitoxin

Protein

• Length: 204 a.a.
• Molecular weight: 22525.50 Da
• Isoelectric Point: 5.3614

>AT66089 WP_096075978.1 NZ_CP017156:175196-175807 [Macrococcus sp. IME1552]
MAMNFKIFEDKLTVSNYVADLFRKQMSNNPTSIIATALGDEAGHVIAELKANIARNPVETSQIHIFDYDKLRGDYGTVGI
VDKQYHEATGKDIMELIKDEAKTKENKGKLTTLFATITQDGSVGYKEINQDNDKGLRSAREIIIVLTGADKASIVEKLYN
TEAGGNFEAANLKTHRMVNVILDDAAAAGLPQDIREYYFQKFA

Nucleotide

• Length: 612 bp

>AT66089 NZ_CP017156:175196-175807 [Macrococcus sp. IME1552]
ATGGCAATGAATTTTAAAATTTTTGAAGATAAATTAACCGTATCGAACTACGTTGCTGATTTATTTAGAAAGCAGATGAG
TAACAATCCGACATCAATTATCGCGACTGCTTTAGGTGATGAAGCAGGTCACGTCATTGCAGAATTAAAGGCTAATATCG
CTCGTAATCCAGTTGAGACGAGTCAAATTCATATCTTTGACTATGATAAATTACGTGGTGATTATGGCACTGTTGGTATC
GTTGATAAACAATACCATGAAGCAACAGGTAAAGATATTATGGAATTGATTAAAGACGAAGCGAAGACTAAAGAGAACAA
AGGGAAGTTGACAACTTTATTTGCTACGATTACACAAGATGGCTCTGTCGGTTATAAAGAGATTAATCAGGATAACGATA
AAGGTTTACGTTCAGCACGTGAAATTATTATCGTATTAACAGGTGCAGACAAAGCTTCAATCGTTGAAAAACTATACAAT
ACTGAAGCGGGTGGCAACTTTGAAGCAGCGAATTTAAAAACACATCGCATGGTCAATGTTATCTTAGATGATGCAGCAGC
AGCTGGATTACCTCAAGATATACGTGAATACTACTTCCAAAAATTTGCGTAA