Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 4649707..4650263 Replicon chromosome
Accession NZ_CP017147
Organism Bosea vaviloviae strain Vaf18

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7U5I4
Locus tag BHK69_RS21335 Protein ID WP_069691854.1
Coordinates 4649707..4650069 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7U5I2
Locus tag BHK69_RS21340 Protein ID WP_069691855.1
Coordinates 4650066..4650263 (-) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHK69_RS21320 4645798..4646877 + 1080 WP_083269596.1 LacI family DNA-binding transcriptional regulator -
BHK69_RS21325 4647176..4648147 + 972 WP_069691852.1 Gfo/Idh/MocA family oxidoreductase -
BHK69_RS21330 4648310..4649662 + 1353 WP_069691853.1 serine hydrolase -
BHK69_RS21335 4649707..4650069 - 363 WP_069691854.1 PIN domain-containing protein Toxin
BHK69_RS21340 4650066..4650263 - 198 WP_069691855.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BHK69_RS21345 4650633..4651595 + 963 WP_148663695.1 OmpA family protein -
BHK69_RS21350 4651737..4652102 + 366 WP_148663530.1 hypothetical protein -
BHK69_RS21355 4652116..4652775 - 660 WP_069691858.1 ceramidase domain-containing protein -
BHK69_RS21360 4652999..4654297 + 1299 WP_069691859.1 GGDEF domain-containing protein -
BHK69_RS21365 4654335..4654595 + 261 WP_069693855.1 YARHG domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-46)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13056.32 Da        Isoelectric Point: 8.9829

>T66076 WP_069691854.1 NZ_CP017147:c4650069-4649707 [Bosea vaviloviae]
VILVDTSVWIDHFRRADAKLGALLEAGQVLGHPFVTAELALGTLRQRSVVIDALQGLPQAAVATHAELLLLIERERLAGI
GIGHVDAHLIAATRLMGDARLWTRDKRLEAVAQRMKLAIT

Download         Length: 363 bp

>T66076 NZ_CP017147:c4650069-4649707 [Bosea vaviloviae]
GTGATCCTGGTCGATACCTCCGTCTGGATCGATCACTTCCGCCGGGCCGATGCGAAGCTCGGCGCCCTGCTCGAGGCCGG
ACAGGTCCTGGGCCACCCGTTCGTGACTGCCGAGCTCGCGCTCGGCACGTTGCGTCAACGCTCTGTCGTCATCGACGCAT
TGCAGGGCTTGCCGCAGGCGGCGGTCGCGACGCACGCCGAGCTTCTGCTGCTGATCGAGCGCGAGAGATTGGCCGGGATA
GGGATCGGCCATGTCGACGCCCATCTCATCGCCGCGACGCGCTTGATGGGGGATGCGCGATTATGGACGCGTGACAAGAG
GCTGGAGGCGGTGGCGCAGCGGATGAAGCTGGCGATCACCTAG

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 7064.02 Da        Isoelectric Point: 10.3690

>AT66076 WP_069691855.1 NZ_CP017147:c4650263-4650066 [Bosea vaviloviae]
MRTTVALDDELLAKAQAYTGLRERSALLREALTALIERESARRLARLGGSDTAAEAAPRRRPDAA

Download         Length: 198 bp

>AT66076 NZ_CP017147:c4650263-4650066 [Bosea vaviloviae]
ATGCGGACGACCGTGGCGCTGGACGATGAATTGCTCGCCAAGGCGCAAGCCTATACGGGCCTGCGTGAGCGCAGCGCGCT
GCTGCGCGAGGCGCTGACGGCGCTGATCGAGCGTGAAAGTGCTAGGCGGCTTGCGCGACTTGGCGGATCGGACACGGCGG
CTGAAGCCGCGCCGCGCCGGCGGCCCGACGCCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7U5I4

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7U5I2

References