Detailed information of TA system TA066075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 4560914..4561559
• Replicon: chromosome
• Accession: NZ_CP017147
• Organism: Bosea vaviloviae strain Vaf18

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BHK69_RS31615
• Protein ID: WP_083269881.1
• Coordinates: 4561227..4561559 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1D7U5B7
• Locus tag: BHK69_RS20895
• Protein ID: WP_069691777.1
• Coordinates: 4560914..4561234 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHK69_RS20875	4557755..4558096	-	342	WP_069693843.1	ATP-dependent Clp protease adapter ClpS	-
BHK69_RS20880	4558395..4559264	+	870	WP_069691774.1	3-deoxy-8-phosphooctulonate synthase	-
BHK69_RS20885	4559350..4560459	+	1110	WP_158516253.1	MFS transporter	-
BHK69_RS20890	4560462..4560917	-	456	WP_148663523.1	preQ(1) synthase	-
BHK69_RS20895	4560914..4561234	-	321	WP_069691777.1	putative addiction module antidote protein	Antitoxin
BHK69_RS31615	4561227..4561559	-	333	WP_083269881.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BHK69_RS20905	4561671..4562954	+	1284	WP_069691779.1	phosphopyruvate hydratase	-
BHK69_RS32715	4563075..4563773	-	699	WP_069691780.1	hypothetical protein	-
BHK69_RS32720	4563972..4564961	+	990	WP_069691781.1	DMT family transporter	-
BHK69_RS20920	4564884..4565633	-	750	WP_069693844.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (28-99)

Antitoxin

• Uncharacterized protein HI1420 (4-90)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12537.35 Da
• Isoelectric Point: 10.5722

>T66075 WP_083269881.1 NZ_CP017147:c4561559-4561227 [Bosea vaviloviae]
IVFYKLQTVTLHLKQTETFRKWERRLKDAKARAAIAARLFRLANGLTGDVSAVGGGVSEIRIHHGPGYRVYFRRRGDQII
ILLCGGDKSTQERDIRAAKQLAAEWEDRDD

Nucleotide

• Length: 333 bp

>T66075 NZ_CP017147:c4561559-4561227 [Bosea vaviloviae]
ATTGTATTTTATAAATTACAAACCGTCACGCTTCATCTCAAGCAGACTGAGACATTTCGCAAATGGGAGCGCAGGCTCAA
GGACGCCAAGGCAAGAGCAGCCATCGCTGCACGCCTCTTTCGTCTCGCCAATGGTCTCACAGGGGACGTCTCCGCAGTCG
GCGGTGGCGTCAGTGAAATCCGCATTCACCACGGCCCCGGCTATCGCGTCTATTTCCGGAGGCGCGGTGACCAGATCATC
ATCCTGCTGTGCGGTGGCGACAAAAGCACGCAAGAGCGCGATATCAGGGCGGCAAAGCAACTGGCTGCGGAATGGGAGGA
TCGGGATGACTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11402.87 Da
• Isoelectric Point: 4.6437

>AT66075 WP_069691777.1 NZ_CP017147:c4561234-4560914 [Bosea vaviloviae]
MTDKLDDYDPADALKSDEAVELFLDDAFASGNARYIAQALGVVARAKGMTKIARETGLAREQLYKSLSENGNPTLETTLA
VLKAIGFEMTGKRHVASPDPLQSLTS

Nucleotide

• Length: 321 bp

>AT66075 NZ_CP017147:c4561234-4560914 [Bosea vaviloviae]
ATGACTGACAAACTCGATGATTACGACCCCGCGGATGCGCTGAAATCCGATGAAGCGGTCGAACTCTTCCTCGACGATGC
CTTTGCCAGCGGCAATGCGCGCTATATCGCGCAGGCGCTTGGCGTCGTCGCTCGTGCGAAGGGAATGACAAAGATCGCTC
GCGAGACCGGGTTGGCGCGCGAGCAGCTCTACAAGTCGCTCAGCGAAAACGGCAATCCGACGCTAGAAACCACATTGGCT
GTGCTAAAGGCGATCGGATTCGAAATGACCGGCAAGCGACATGTCGCATCGCCAGACCCCCTGCAGAGCCTGACATCATG
A

Structures

Antitoxin

• AlphaFold DB: A0A1D7U5B7