Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) brnT-shpB/BrnT_toxin-BrnA
Location 1852996..1853521 Replicon chromosome
Accession NZ_CP017147
Organism Bosea vaviloviae strain Vaf18

Toxin (Protein)


Gene name brnT Uniprot ID A0A1D7TZJ8
Locus tag BHK69_RS08670 Protein ID WP_069689744.1
Coordinates 1853246..1853521 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D7TZH8
Locus tag BHK69_RS08665 Protein ID WP_069689743.1
Coordinates 1852996..1853262 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHK69_RS08640 1848264..1848794 - 531 WP_069693490.1 GNAT family N-acetyltransferase -
BHK69_RS08645 1848818..1849384 - 567 WP_199579216.1 helix-turn-helix domain-containing protein -
BHK69_RS08650 1849621..1850406 + 786 WP_069689741.1 hypothetical protein -
BHK69_RS08655 1850452..1852257 + 1806 WP_069693491.1 translation elongation factor 4 -
BHK69_RS08660 1852286..1852996 - 711 WP_083269822.1 HAD family phosphatase -
BHK69_RS08665 1852996..1853262 - 267 WP_069689743.1 BrnA antitoxin family protein Antitoxin
BHK69_RS08670 1853246..1853521 - 276 WP_069689744.1 BrnT family toxin Toxin
BHK69_RS08675 1853654..1854532 + 879 WP_069693492.1 sulfoxide reductase heme-binding subunit YedZ -
BHK69_RS08680 1854590..1856317 + 1728 WP_083269206.1 MFS transporter -
BHK69_RS08685 1856450..1857247 - 798 WP_069689745.1 undecaprenyl-diphosphate phosphatase -
BHK69_RS08690 1857491..1858183 + 693 WP_069689746.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-83)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(26-80)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10435.80 Da        Isoelectric Point: 5.1875

>T66070 WP_069689744.1 NZ_CP017147:c1853521-1853246 [Bosea vaviloviae]
IPEFEWDPEKDTANVAKHGVAFEFAKRIFEGPVLSWVDDRVDYGEVREISIGRVDGIVVLVVVHTDRDGRIRIISARPVS
QKERQRYEASL

Download         Length: 276 bp

>T66070 NZ_CP017147:c1853521-1853246 [Bosea vaviloviae]
ATACCTGAATTCGAATGGGATCCTGAGAAGGACACGGCCAATGTCGCCAAGCACGGCGTCGCCTTCGAATTTGCGAAACG
CATTTTCGAGGGACCGGTGCTGAGCTGGGTCGATGACCGCGTCGACTATGGCGAGGTTCGAGAGATCAGCATCGGTCGTG
TCGACGGCATCGTTGTGCTCGTCGTCGTGCATACCGACCGCGACGGCCGCATCCGCATCATCTCGGCCCGACCGGTCTCT
CAAAAGGAAAGGCAGCGCTATGAAGCCTCCCTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10115.55 Da        Isoelectric Point: 8.3958

>AT66070 WP_069689743.1 NZ_CP017147:c1853262-1852996 [Bosea vaviloviae]
MKPPYDKPLSPEALAALKDDEIDFSDIPELDETFWKNAKLVEPDRTQLVTLRVKKSVLEAYKAQGRGYQTRMNAVLETYA
RSQLKRTS

Download         Length: 267 bp

>AT66070 NZ_CP017147:c1853262-1852996 [Bosea vaviloviae]
ATGAAGCCTCCCTATGACAAACCGCTGTCGCCTGAGGCTCTCGCCGCGCTCAAGGACGACGAAATCGATTTCAGCGACAT
CCCCGAGCTGGACGAGACCTTCTGGAAAAACGCGAAGCTGGTCGAGCCCGACCGGACGCAACTGGTGACTCTGCGCGTCA
AGAAATCGGTCCTGGAAGCCTATAAGGCCCAGGGCCGCGGCTATCAGACCCGCATGAACGCCGTTCTCGAAACCTATGCC
CGTTCCCAACTCAAGCGGACTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7TZJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7TZH8

References