Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 348507..349147 Replicon chromosome
Accession NZ_CP017143
Organism Aeromonas salmonicida subsp. masoucida strain RFAS1

Toxin (Protein)


Gene name vagD Uniprot ID A0A807Z4U5
Locus tag BHG40_RS01765 Protein ID WP_005320982.1
Coordinates 348734..349147 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A807Z5T0
Locus tag BHG40_RS01760 Protein ID WP_005320985.1
Coordinates 348507..348737 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHG40_RS01735 344483..345874 + 1392 WP_034283822.1 T3SS effector protein-tyrosine-phosphatase AopH -
BHG40_RS01740 345947..346348 - 402 Protein_311 IS4 family transposase -
BHG40_RS01750 346416..347333 + 918 WP_076611341.1 IS5-like element ISAs4 family transposase -
BHG40_RS23640 347750..348139 + 390 WP_139403308.1 site-specific integrase -
BHG40_RS01755 348052..348438 + 387 WP_017413139.1 tyrosine-type recombinase/integrase -
BHG40_RS01760 348507..348737 + 231 WP_005320985.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BHG40_RS01765 348734..349147 + 414 WP_005320982.1 PIN domain-containing protein Toxin
BHG40_RS01770 349225..349578 + 354 WP_017413138.1 hypothetical protein -
BHG40_RS01775 349605..349880 + 276 Protein_318 hypothetical protein -
BHG40_RS01780 349938..350855 - 918 WP_076611341.1 IS5-like element ISAs4 family transposase -
BHG40_RS01785 351090..352007 - 918 WP_088814123.1 IS5-like element ISAs4 family transposase -
BHG40_RS01790 352194..352775 - 582 Protein_321 subclass B2 metallo-beta-lactamase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - aopH 336534..352007 15473
- inside IScluster/Tn - aopH 336534..354243 17709


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-42)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.7146

>T66064 WP_005320982.1 NZ_CP017143:348734-349147 [Aeromonas salmonicida subsp. masoucida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Download         Length: 414 bp

>T66064 NZ_CP017143:348734-349147 [Aeromonas salmonicida subsp. masoucida]
GTGAAGACGTTTATGCTCGATACCTGCATTTGTTCCTTCATCATGCGCGAAATGCCACAGTCAGTTCTTGAACGGTTGGG
CGCAGAGGTGGCGAGCGGCAATCGGATTGTGATCTCGGCAATCACCTACGCCGAAATGCGTTATGGCCAGATCGGCAAGA
AGGCTTCTCCGAAGCTGGGGCCAGCCATTGATGAATTTGTTCGTCGTCTCGATGGGATCTTGCCATGGGACGCTGCTACG
GTCGATCAGACGCTCGAAGTGCGCCAGCAGTTAGCAGCCTTGGGCACTCCGATCGGCAATAACGATGCTGCTATTGCCGC
CCACGCCCTGACGGCTGGCTGTGTACTGGTAACCAACAATACCCGCGAGTTTTCCCGAGTGGCGGGGCTGCTCTATGAAG
ACTGGGTTCACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8699.88 Da        Isoelectric Point: 4.7419

>AT66064 WP_005320985.1 NZ_CP017143:348507-348737 [Aeromonas salmonicida subsp. masoucida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Download         Length: 231 bp

>AT66064 NZ_CP017143:348507-348737 [Aeromonas salmonicida subsp. masoucida]
ATGCGTACTGTATCAATTTTTAAAAATGGTAAAAATCAGGCGGTGAGACTGCCAAAAGACATGGAGTTTCAGGGGGTTAA
TGACCTTGAGATTATCAAAAATGGCGACACAATCACGTTGCGCCCGGCCCGCCCTGATTGGTTTTCCCTGGCGGAATGCC
AGAAGGTGGATGCAGATTTCTTGCAAGAACGTACCGATGTGATCAGCGATGAAGGACGGTTCGCCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807Z4U5

Antitoxin

Source ID Structure
AlphaFold DB A0A807Z5T0

References