Detailed information of TA system TA066042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2134155..2134684
• Replicon: chromosome
• Accession: NZ_CP017115
• Organism: Staphylococcus aureus strain FORC_045

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FORC45_RS11105
• Protein ID: WP_000621175.1
• Coordinates: 2134155..2134517 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FORC45_RS11110
• Protein ID: WP_000948331.1
• Coordinates: 2134514..2134684 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC45_RS11080	2131134..2131904	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
FORC45_RS11085	2131879..2132358	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
FORC45_RS11090	2132360..2132686	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FORC45_RS11095	2132805..2133806	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FORC45_RS11105	2134155..2134517	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FORC45_RS11110	2134514..2134684	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FORC45_RS11115	2134769..2135917	-	1149	WP_001281154.1	alanine racemase	-
FORC45_RS11120	2135983..2136342	-	360	WP_000581197.1	holo-ACP synthase	-
FORC45_RS11125	2136346..2136837	-	492	WP_001205912.1	PH domain-containing protein	-
FORC45_RS11130	2136824..2138407	-	1584	WP_001294626.1	PH domain-containing protein	-
FORC45_RS11135	2138400..2138879	-	480	WP_001287078.1	hypothetical protein	-
FORC45_RS11140	2139088..2139648	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T66042 WP_000621175.1 NZ_CP017115:c2134517-2134155 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T66042 NZ_CP017115:c2134517-2134155 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT66042 WP_000948331.1 NZ_CP017115:c2134684-2134514 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT66042 NZ_CP017115:c2134684-2134514 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1