66018

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1326569..1327485 Replicon chromosome
Accession NZ_CP017112
Organism Bacillus subtilis strain BS16045

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag BS16045_RS07030 Protein ID WP_003244695.1
Coordinates 1326739..1327485 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag BS16045_RS07025 Protein ID WP_003232646.1
Coordinates 1326569..1326739 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS16045_RS06995 1323432..1323761 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
BS16045_RS07000 1323758..1323922 + 165 WP_003232658.1 XkdX family protein -
BS16045_RS07005 1323966..1324805 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
BS16045_RS07010 1324858..1325127 + 270 WP_003232655.1 hemolysin XhlA family protein -
BS16045_RS07015 1325140..1325403 + 264 WP_003232653.1 phage holin -
BS16045_RS07020 1325416..1326309 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
BS16045_RS22305 1326346..1326483 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
BS16045_RS07025 1326569..1326739 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
BS16045_RS07030 1326739..1327485 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
BS16045_RS07035 1327595..1328596 - 1002 WP_003232642.1 inorganic phosphate transporter -
BS16045_RS07040 1328609..1329226 - 618 WP_003218470.1 DUF47 domain-containing protein -
BS16045_RS07045 1329502..1330818 - 1317 WP_003244819.1 serine/threonine exchanger -
BS16045_RS07050 1331207..1332157 + 951 WP_003245086.1 ring-cleaving dioxygenase -
BS16045_RS22310 1332258..1332404 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1292588..1335905 43317


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T66018 WP_003244695.1 NZ_CP017112:c1327485-1326739 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T66018 NZ_CP017112:c1327485-1326739 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT66018 WP_003232646.1 NZ_CP017112:c1326739-1326569 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT66018 NZ_CP017112:c1326739-1326569 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References