Detailed information of TA system TA066015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 637882..638387
• Replicon: chromosome
• Accession: NZ_CP017111
• Organism: Sulfurospirillum halorespirans DSM 13726 strain PCE-M2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A1D7THD5
• Locus tag: SHALO_RS03185
• Protein ID: WP_025343819.1
• Coordinates: 638127..638387 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1D7THA9
• Locus tag: SHALO_RS03180
• Protein ID: WP_025343818.1
• Coordinates: 637882..638130 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHALO_RS03140	633598..633891	+	294	WP_174543312.1	nucleotidyltransferase family protein	-
SHALO_RS03145	633884..634219	+	336	WP_069477340.1	DUF86 domain-containing protein	-
SHALO_RS03150	634423..635754	+	1332	WP_069477341.1	OprD family outer membrane porin	-
SHALO_RS03155	635918..636199	+	282	WP_025343812.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SHALO_RS03160	636186..636509	+	324	WP_025343813.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SHALO_RS03165	636865..637062	+	198	WP_025343815.1	DUF2283 domain-containing protein	-
SHALO_RS03170	637298..637513	+	216	WP_025343816.1	CopG family transcriptional regulator	-
SHALO_RS03175	637503..637757	+	255	WP_025343817.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SHALO_RS03180	637882..638130	+	249	WP_025343818.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SHALO_RS03185	638127..638387	+	261	WP_025343819.1	Txe/YoeB family addiction module toxin	Toxin
SHALO_RS03190	638514..639788	+	1275	WP_069477342.1	OprD family outer membrane porin	-
SHALO_RS03195	639947..640237	+	291	WP_025343821.1	nucleotidyltransferase domain-containing protein	-
SHALO_RS03200	640455..640709	+	255	WP_145923217.1	hypothetical protein	-
SHALO_RS03205	641003..642121	+	1119	WP_037961242.1	ABC transporter substrate-binding protein	-
SHALO_RS03210	642234..643118	+	885	WP_038533775.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10353.99 Da
• Isoelectric Point: 9.3793

>T66015 WP_025343819.1 NZ_CP017111:638127-638387 [Sulfurospirillum halorespirans DSM 13726]
MIIGFAAKGWEDYLYWQENDKKIVKRINLLLEDIKRNPHDSNGIGKPERLKGDLEKYFSRRITAEHRLVYKFLDDLIIVA
QCRFHY

Nucleotide

• Length: 261 bp

>T66015 NZ_CP017111:638127-638387 [Sulfurospirillum halorespirans DSM 13726]
ATGATCATTGGCTTTGCCGCGAAAGGATGGGAAGATTATCTTTATTGGCAAGAAAATGATAAAAAAATTGTAAAACGCAT
CAATCTACTTTTAGAAGATATTAAACGAAATCCACATGATAGCAATGGTATTGGAAAACCAGAACGACTAAAAGGTGACT
TGGAAAAATACTTTTCAAGGCGTATTACTGCAGAGCATCGATTGGTTTATAAATTTTTGGATGATTTAATTATTGTGGCA
CAATGCAGATTTCATTATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9486.84 Da
• Isoelectric Point: 4.7246

>AT66015 WP_025343818.1 NZ_CP017111:637882-638130 [Sulfurospirillum halorespirans DSM 13726]
MQAIFYSQARNNLRSIISKACTDFDQFIITTKDNQSVVLMSYDEYSAIKETMYLLSSKTNRDRLLDAVEEIEGMKFTCKE
IA

Nucleotide

• Length: 249 bp

>AT66015 NZ_CP017111:637882-638130 [Sulfurospirillum halorespirans DSM 13726]
ATGCAAGCCATTTTTTATTCACAGGCTAGAAATAACCTAAGAAGTATTATAAGCAAAGCATGCACGGATTTTGATCAGTT
CATCATTACAACAAAAGATAATCAGTCTGTTGTGCTCATGTCCTACGATGAATACAGTGCCATCAAAGAAACAATGTACC
TACTTTCTTCAAAAACTAATAGAGATAGGCTCTTAGATGCTGTTGAAGAGATTGAAGGTATGAAATTTACATGTAAAGAA
ATTGCATGA

Structures

Toxin

• AlphaFold DB: A0A1D7THD5

Antitoxin

• AlphaFold DB: A0A1D7THA9