Detailed information of TA system TA066010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2006772..2007562
• Replicon: plasmid pRgalIE4872d
• Accession: NZ_CP017105
• Organism: Rhizobium gallicum strain IE4872

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A1L5NX53
• Locus tag: IE4872_RS34815
• Protein ID: WP_074072674.1
• Coordinates: 2006772..2007269 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: IE4872_RS34820
• Protein ID: WP_074072675.1
• Coordinates: 2007266..2007562 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE4872_RS34780	2002464..2003171	-	708	WP_074072668.1	5-oxoprolinase subunit PxpB	-
IE4872_RS34785	2003168..2003596	-	429	WP_074072669.1	acetyl-CoA carboxylase	-
IE4872_RS34790	2003593..2004984	-	1392	WP_074072670.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
IE4872_RS34795	2004984..2005427	-	444	WP_074072671.1	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	-
IE4872_RS34805	2006068..2006268	+	201	WP_074073155.1	type II toxin-antitoxin system VapB family antitoxin	-
IE4872_RS34810	2006265..2006648	+	384	WP_074072673.1	type II toxin-antitoxin system VapC family toxin	-
IE4872_RS34815	2006772..2007269	-	498	WP_074072674.1	GNAT family N-acetyltransferase	Toxin
IE4872_RS34820	2007266..2007562	-	297	WP_074072675.1	DUF1778 domain-containing protein	Antitoxin
IE4872_RS34825	2008089..2008562	+	474	WP_074073156.1	VOC family protein	-
IE4872_RS34830	2008688..2009680	+	993	WP_074072676.1	hypothetical protein	-
IE4872_RS34835	2009731..2009943	-	213	WP_074072677.1	hypothetical protein	-
IE4872_RS34840	2010009..2010608	-	600	WP_074072678.1	hypothetical protein	-
IE4872_RS34845	2010872..2011888	+	1017	WP_074072679.1	taurine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA / katA / htpB	1..2388366	2388366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (34-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-90)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17840.52 Da
• Isoelectric Point: 8.0536

>T66010 WP_074072674.1 NZ_CP017105:c2007269-2006772 [Rhizobium gallicum]
VTLSAPTPLADHHELAEFISGVVELDEWLRRRARANQAGGASRTFVVCEGNRVIAYYALASGAVKEPEAPGRFRRNMPDP
IPVAVLGRLAIDRTYQGRGLGRALVRDAGLRLLNAAEILGIRGVLVHAISDDARAFYEAVGFLPSPSDPMMLMVGLRDLN
NALNP

Nucleotide

• Length: 498 bp

>T66010 NZ_CP017105:c2007269-2006772 [Rhizobium gallicum]
GTGACCCTCAGCGCACCGACACCACTGGCCGACCATCACGAACTGGCGGAATTCATTTCCGGCGTCGTTGAACTGGACGA
GTGGCTGCGTCGCCGCGCCCGCGCCAATCAGGCAGGCGGCGCATCGCGCACATTCGTCGTGTGCGAGGGCAACCGCGTGA
TCGCCTATTATGCGCTTGCCTCCGGCGCCGTTAAAGAGCCGGAAGCACCTGGCCGTTTCCGACGCAACATGCCCGACCCG
ATTCCGGTTGCCGTTCTCGGCCGCCTCGCCATTGACCGGACCTATCAGGGGCGCGGTCTCGGCAGGGCATTGGTGCGCGA
TGCGGGCCTGCGTCTGCTCAATGCCGCTGAAATCCTCGGTATCCGCGGCGTGTTGGTACATGCGATTTCCGATGATGCGC
GAGCCTTCTATGAGGCAGTCGGTTTTCTGCCTTCGCCGTCCGACCCCATGATGCTGATGGTTGGGCTGCGTGATTTGAAC
AACGCGCTGAATCCCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11070.61 Da
• Isoelectric Point: 7.0159

>AT66010 WP_074072675.1 NZ_CP017105:c2007562-2007266 [Rhizobium gallicum]
MPTSHTSKSADASVPLNIRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIFTVSPEIYAEYLARLDAPAQ
PNERLKRTMTTKAPWDEA

Nucleotide

• Length: 297 bp

>AT66010 NZ_CP017105:c2007562-2007266 [Rhizobium gallicum]
ATGCCCACATCCCACACCAGTAAAAGCGCAGATGCCAGCGTGCCGCTGAATATCCGCATCAAGCCGGCGACCCGAAACCT
TATCGATCGCGCCGCCGAGCTGCTTGGCAAGACGCGCACGGATTTCATGCTGGAGGCTTCCGAGCGCCGAGCCGAGGAAG
TTCTGCTCGACCGTACGATATTCACGGTTAGTCCCGAAATCTATGCCGAGTACCTTGCCCGGCTCGATGCGCCCGCCCAA
CCCAATGAGCGACTGAAACGTACTATGACGACCAAGGCGCCGTGGGATGAGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A1L5NX53