Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2006068..2006648 Replicon plasmid pRgalIE4872d
Accession NZ_CP017105
Organism Rhizobium gallicum strain IE4872

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L5NX89
Locus tag IE4872_RS34810 Protein ID WP_074072673.1
Coordinates 2006265..2006648 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L5NX67
Locus tag IE4872_RS34805 Protein ID WP_074073155.1
Coordinates 2006068..2006268 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE4872_RS34775 2001472..2002467 - 996 WP_074072667.1 biotin-dependent carboxyltransferase family protein -
IE4872_RS34780 2002464..2003171 - 708 WP_074072668.1 5-oxoprolinase subunit PxpB -
IE4872_RS34785 2003168..2003596 - 429 WP_074072669.1 acetyl-CoA carboxylase -
IE4872_RS34790 2003593..2004984 - 1392 WP_074072670.1 acetyl-CoA carboxylase biotin carboxylase subunit -
IE4872_RS34795 2004984..2005427 - 444 WP_074072671.1 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit -
IE4872_RS34805 2006068..2006268 + 201 WP_074073155.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IE4872_RS34810 2006265..2006648 + 384 WP_074072673.1 type II toxin-antitoxin system VapC family toxin Toxin
IE4872_RS34815 2006772..2007269 - 498 WP_074072674.1 GNAT family N-acetyltransferase -
IE4872_RS34820 2007266..2007562 - 297 WP_074072675.1 DUF1778 domain-containing protein -
IE4872_RS34825 2008089..2008562 + 474 WP_074073156.1 VOC family protein -
IE4872_RS34830 2008688..2009680 + 993 WP_074072676.1 hypothetical protein -
IE4872_RS34835 2009731..2009943 - 213 WP_074072677.1 hypothetical protein -
IE4872_RS34840 2010009..2010608 - 600 WP_074072678.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA / katA / htpB 1..2388366 2388366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13998.13 Da        Isoelectric Point: 6.7544

>T66009 WP_074072673.1 NZ_CP017105:2006265-2006648 [Rhizobium gallicum]
VILADTSIWIDHFRHVDAELRRIIEDDRLLCHPTVIGELALGSLRDRGSVIAFLAAQRGAVVATQDEVMTMIDRHGIFSM
GIGYTDAHLLASVLLDQRAALWTRNKRLQGAAEKAGASLYIPVNLSH

Download         Length: 384 bp

>T66009 NZ_CP017105:2006265-2006648 [Rhizobium gallicum]
GTGATCCTAGCGGACACCTCGATCTGGATTGATCATTTCCGCCATGTCGATGCCGAGCTGCGCAGGATTATTGAGGACGA
TCGCCTACTTTGCCACCCGACCGTGATCGGCGAGCTTGCGCTCGGCAGTCTTCGGGATCGCGGCAGTGTAATTGCATTTC
TGGCGGCGCAGCGCGGAGCGGTTGTCGCCACCCAAGACGAAGTCATGACAATGATCGATCGGCACGGCATTTTCAGCATG
GGCATTGGCTACACGGATGCGCACTTGCTGGCATCCGTCCTTCTTGACCAGCGAGCGGCCTTATGGACGAGAAACAAGCG
CCTGCAGGGAGCGGCCGAAAAGGCGGGCGCTTCACTTTATATACCGGTCAACCTGTCCCACTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7138.30 Da        Isoelectric Point: 11.1107

>AT66009 WP_074073155.1 NZ_CP017105:2006068-2006268 [Rhizobium gallicum]
MRSTINLDDMLLEKARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSTAAK

Download         Length: 201 bp

>AT66009 NZ_CP017105:2006068-2006268 [Rhizobium gallicum]
ATGCGATCGACTATTAACCTCGACGACATGCTTTTGGAAAAGGCTAGGTCCTTAACCGGTACAAAGGAAACCGCTGCGCT
CGTCCGCCAGGCGTTGGAGACGCTTGTTCGTGTGGAGTCCGGAAAGCGCCTCATCGCGCTCGGAGGAACCATGCCCGATG
CCGAGGCAGCCCCGCGCCGCCGGAGCACAGCAGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5NX89

Antitoxin

Source ID Structure
AlphaFold DB A0A1L5NX67

References