Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1148159..1148733 Replicon chromosome
Accession NZ_CP017101
Organism Rhizobium gallicum strain IE4872

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L5NFT3
Locus tag IE4872_RS05735 Protein ID WP_074066968.1
Coordinates 1148368..1148733 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L5NFX2
Locus tag IE4872_RS05730 Protein ID WP_074066967.1
Coordinates 1148159..1148374 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE4872_RS05705 1144370..1145323 - 954 WP_022716380.1 sulfate adenylyltransferase subunit CysD -
IE4872_RS05710 1145483..1146223 - 741 WP_074066964.1 phosphoadenylyl-sulfate reductase -
IE4872_RS05715 1146647..1147093 + 447 WP_074066965.1 Rrf2 family transcriptional regulator -
IE4872_RS05720 1147233..1147619 + 387 WP_028739821.1 hypothetical protein -
IE4872_RS05725 1147624..1148100 + 477 WP_074066966.1 DUF2214 domain-containing protein -
IE4872_RS05730 1148159..1148374 + 216 WP_074066967.1 ribbon-helix-helix domain-containing protein Antitoxin
IE4872_RS05735 1148368..1148733 + 366 WP_074066968.1 type II toxin-antitoxin system VapC family toxin Toxin
IE4872_RS05740 1149167..1150816 - 1650 WP_074066969.1 choline dehydrogenase -
IE4872_RS05745 1150876..1152339 - 1464 WP_074066970.1 betaine-aldehyde dehydrogenase -
IE4872_RS05750 1152404..1152988 - 585 WP_074066971.1 transcriptional regulator BetI -
IE4872_RS05755 1153131..1153685 + 555 WP_074066972.1 HdeD family acid-resistance protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13654.71 Da        Isoelectric Point: 6.4703

>T66005 WP_074066968.1 NZ_CP017101:1148368-1148733 [Rhizobium gallicum]
MVKALFDTNILIDYLNAIPEARDELDRYGRRAISVVTWMEVLIGADPDVEAATRSFLNNFQIIAVDNAIAEGAVRLRQRH
RIKLPDAIIWSTADAHALLLVTRNTKDFPADNPGIRVPYLR

Download         Length: 366 bp

>T66005 NZ_CP017101:1148368-1148733 [Rhizobium gallicum]
ATGGTGAAAGCGCTCTTCGATACGAACATCCTTATCGACTATCTCAACGCAATACCGGAAGCCCGCGACGAGCTTGACCG
CTATGGACGCCGTGCCATCAGCGTCGTCACTTGGATGGAGGTGTTGATTGGCGCTGATCCCGACGTCGAAGCAGCGACGC
GAAGCTTCTTGAATAACTTCCAGATCATCGCGGTTGATAATGCGATAGCGGAAGGCGCTGTTCGCTTAAGACAGCGCCAC
CGGATCAAATTGCCGGACGCGATAATCTGGTCGACCGCCGATGCGCATGCGTTGCTCCTGGTGACGCGCAACACCAAGGA
CTTTCCGGCGGATAATCCTGGAATCCGCGTCCCATATCTGCGTTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8288.48 Da        Isoelectric Point: 8.6003

>AT66005 WP_074066967.1 NZ_CP017101:1148159-1148374 [Rhizobium gallicum]
MRILVDIVESELKALDKLAKSEKVSRASLIRKAVNDFLDRHDRTKEAEAFGLWGNRKIDGLEYQDKVRSEW

Download         Length: 216 bp

>AT66005 NZ_CP017101:1148159-1148374 [Rhizobium gallicum]
ATGCGTATCCTGGTCGATATCGTCGAATCCGAGTTGAAGGCGCTGGATAAGTTGGCGAAGAGCGAGAAGGTTTCCCGCGC
TTCGCTCATCCGCAAAGCTGTCAACGATTTTCTGGATCGTCATGATCGGACAAAAGAGGCAGAAGCTTTCGGGCTCTGGG
GTAATAGAAAGATCGATGGGCTCGAATACCAGGATAAAGTTCGAAGCGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5NFT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1L5NFX2

References