Detailed information of TA system TA066000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4474450..4475264
• Replicon: chromosome
• Accession: NZ_CP017100
• Organism: Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: NEB5A_RS22170
• Protein ID: WP_001054376.1
• Coordinates: 4475007..4475264 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: NEB5A_RS22165
• Protein ID: WP_001309181.1
• Coordinates: 4474450..4474995 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NEB5A_RS22145	4470141..4471454	-	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-
NEB5A_RS22150	4471466..4471744	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
NEB5A_RS22155	4471741..4472862	-	1122	WP_000010829.1	M42 family metallopeptidase	-
NEB5A_RS25700	4473107..4473223	-	117	Protein_4217	VOC family protein	-
NEB5A_RS24935	4473261..4473479	-	219	Protein_4218	hypothetical protein	-
NEB5A_RS22160	4473648..4474394	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
NEB5A_RS22165	4474450..4474995	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
NEB5A_RS22170	4475007..4475264	-	258	WP_001054376.1	hypothetical protein	Toxin
NEB5A_RS25390	4475641..4475886	-	246	Protein_4222	GNAT family N-acetyltransferase	-
NEB5A_RS22185	4476002..4477242	+	1241	Protein_4223	helicase YjhR	-
NEB5A_RS22190	4477825..4478805	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
NEB5A_RS22195	4478870..4479976	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4471741..4484620	12879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T66000 WP_001054376.1 NZ_CP017100:c4475264-4475007 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T66000 NZ_CP017100:c4475264-4475007 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT66000 WP_001309181.1 NZ_CP017100:c4474995-4474450 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT66000 NZ_CP017100:c4474995-4474450 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8