Detailed information of TA system
Overview
TA module
| Type | VII | Classification (family/domain) | HepT-MntA/HEPN-MNT |
| Location | 3301651..3302468 | Replicon | chromosome |
| Accession | NC_004347 | ||
| Organism | Shewanella oneidensis MR-1 | ||
Toxin (Protein)
| Gene name | hepT | Uniprot ID | Q8ECH6 |
| Locus tag | SO_RS14755 | Protein ID | WP_011073053.1 |
| Coordinates | 3302072..3302473 (+) | Length | 134 a.a. |
Antitoxin (Protein)
| Gene name | mntA | Uniprot ID | Q8ECH7 |
| Locus tag | SO_RS14750 | Protein ID | WP_011073052.1 |
| Coordinates | 3301656..3302075 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SO_RS14725 (SO_3160) | 3297552..3298091 | - | 540 | WP_011073048.1 | dTDP-4-dehydrorhamnose 3,5-epimerase | - |
| SO_RS14730 (SO_3161) | 3298078..3298260 | - | 183 | Protein_2853 | glucose-1-phosphate thymidylyltransferase | - |
| SO_RS14735 (SO_3162) | 3298465..3299826 | - | 1362 | WP_011073049.1 | GAF domain-containing sensor histidine kinase | - |
| SO_RS14740 (SO_3163) | 3300043..3300489 | + | 447 | WP_164925739.1 | copper resistance protein NlpE | - |
| SO_RS14745 (SO_3164) | 3300783..3301550 | + | 768 | WP_011073051.1 | HutD family protein | - |
| SO_RS14750 (SO_3165) | 3301656..3302075 | + | 420 | WP_011073052.1 | type II toxin-antitoxin system antitoxin | Antitoxin |
| SO_RS14755 (SO_3166) | 3302072..3302473 | + | 402 | WP_011073053.1 | type II toxin-antitoxin system toxin | Toxin |
| SO_RS14760 (SO_3167) | 3302667..3303698 | - | 1032 | WP_011073054.1 | dTDP-glucose 4,6-dehydratase | - |
| SO_RS14765 (SO_3168) | 3303962..3305161 | - | 1200 | WP_164925891.1 | molecular chaperone DnaJ | - |
| SO_RS14770 (SO_3169) | 3305365..3305757 | - | 393 | WP_164925740.1 | helix-turn-helix domain-containing protein | - |
| SO_RS14775 (SO_3170) | 3305742..3307109 | - | 1368 | WP_011073057.1 | HipA domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 134 a.a. Molecular weight: 15312.57 Da Isoelectric Point: 6.3185
>T660 WP_011073053.1 NC_004347:3302072-3302473 [Shewanella oneidensis MR-1]
MNDIIINKIATIKRCIKRIQQVYGDGSQFKQDFTLQDSVILNLQRCCEACIDIANHINRQQQLGIPQSSRDSFTLLAQNN
LITQPLSDNLKKMVGLRNIAVHDYQELNLDIVVHVVQHHLEDFEQFIDVIKAE
MNDIIINKIATIKRCIKRIQQVYGDGSQFKQDFTLQDSVILNLQRCCEACIDIANHINRQQQLGIPQSSRDSFTLLAQNN
LITQPLSDNLKKMVGLRNIAVHDYQELNLDIVVHVVQHHLEDFEQFIDVIKAE
Download Length: 402 bp
>T660 NC_004347:3302072-3302473 [Shewanella oneidensis MR-1]
TTGAATGACATTATTATCAATAAAATAGCCACCATCAAACGCTGTATAAAGCGCATCCAACAAGTCTATGGTGATGGCAG
CCAGTTCAAACAAGACTTCACCTTGCAAGATAGCGTTATCCTCAATTTACAACGCTGCTGCGAAGCCTGTATCGACATAG
CCAATCATATTAACCGCCAACAACAACTAGGCATCCCCCAAAGTAGCCGTGATAGCTTTACATTACTCGCACAAAATAAC
CTTATTACTCAGCCACTCTCGGATAACTTAAAGAAAATGGTCGGCCTGCGAAACATCGCAGTACATGACTACCAAGAACT
CAACCTCGATATCGTTGTGCATGTTGTACAGCATCATCTTGAGGATTTTGAGCAATTTATTGATGTGATAAAAGCAGAGT
GA
TTGAATGACATTATTATCAATAAAATAGCCACCATCAAACGCTGTATAAAGCGCATCCAACAAGTCTATGGTGATGGCAG
CCAGTTCAAACAAGACTTCACCTTGCAAGATAGCGTTATCCTCAATTTACAACGCTGCTGCGAAGCCTGTATCGACATAG
CCAATCATATTAACCGCCAACAACAACTAGGCATCCCCCAAAGTAGCCGTGATAGCTTTACATTACTCGCACAAAATAAC
CTTATTACTCAGCCACTCTCGGATAACTTAAAGAAAATGGTCGGCCTGCGAAACATCGCAGTACATGACTACCAAGAACT
CAACCTCGATATCGTTGTGCATGTTGTACAGCATCATCTTGAGGATTTTGAGCAATTTATTGATGTGATAAAAGCAGAGT
GA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15566.64 Da Isoelectric Point: 4.8616
>AT660 WP_011073052.1 NC_004347:3301656-3302075 [Shewanella oneidensis MR-1]
MQQLNENKIIKLLRDNIPKLQLIYLFGSYSQGTQHRNSDIDIAVLAADTLDNIARWELAQKLASALDSDVDLVDLRSAST
VLCQQVVTQGKQLWGTQQDDELFAVKTISMYQHLQAERQAIIDDVMANTAAKAHRGESL
MQQLNENKIIKLLRDNIPKLQLIYLFGSYSQGTQHRNSDIDIAVLAADTLDNIARWELAQKLASALDSDVDLVDLRSAST
VLCQQVVTQGKQLWGTQQDDELFAVKTISMYQHLQAERQAIIDDVMANTAAKAHRGESL
Download Length: 420 bp
>AT660 NC_004347:3301656-3302075 [Shewanella oneidensis MR-1]
ATGCAACAACTAAATGAAAATAAAATCATAAAGTTACTGCGAGACAACATCCCAAAGTTGCAATTAATCTACCTGTTTGG
CTCTTATTCGCAGGGAACACAACACCGCAACAGCGATATCGATATCGCGGTACTCGCCGCTGACACTTTAGACAATATTG
CACGCTGGGAACTTGCCCAAAAACTCGCTTCGGCGTTGGACTCAGATGTTGATCTGGTTGATTTACGCTCAGCATCCACC
GTGTTATGCCAACAGGTAGTCACTCAAGGGAAGCAACTTTGGGGAACACAACAAGATGATGAACTTTTTGCCGTAAAGAC
CATCTCAATGTACCAACATCTACAAGCCGAAAGACAGGCAATTATTGATGATGTAATGGCCAACACCGCAGCCAAAGCTC
ATAGGGGGGAGTCACTTTGA
ATGCAACAACTAAATGAAAATAAAATCATAAAGTTACTGCGAGACAACATCCCAAAGTTGCAATTAATCTACCTGTTTGG
CTCTTATTCGCAGGGAACACAACACCGCAACAGCGATATCGATATCGCGGTACTCGCCGCTGACACTTTAGACAATATTG
CACGCTGGGAACTTGCCCAAAAACTCGCTTCGGCGTTGGACTCAGATGTTGATCTGGTTGATTTACGCTCAGCATCCACC
GTGTTATGCCAACAGGTAGTCACTCAAGGGAAGCAACTTTGGGGAACACAACAAGATGATGAACTTTTTGCCGTAAAGAC
CATCTCAATGTACCAACATCTACAAGCCGAAAGACAGGCAATTATTGATGATGTAATGGCCAACACCGCAGCCAAAGCTC
ATAGGGGGGAGTCACTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Jianyun Yao et al. (2015) Identification and characterization of a HEPN-MNT family type II toxin-antitoxin in Shewanella oneidensis. Microbial Biotechnology 8(6):961-73. [PubMed:26112399]
(2) Xuanyan Jia et al. (2018) Structure-function analyses reveal the molecular architecture and neutralization mechanism of a bacterial HEPN-MNT toxin-antitoxin system. The Journal of Biological Chemistry 293(18):6812-6823. [PubMed:29555683]
(3) Jianyun Yao et al. (2020) Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/antitoxin system. Nucleic Acids Research 48(19):11054-11067. [PubMed:33045733]