Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4389658..4390253 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag NEB5A_RS21740 Protein ID WP_000239577.1
Coordinates 4389903..4390253 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag NEB5A_RS21735 Protein ID WP_001223208.1
Coordinates 4389658..4389909 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS21725 4385323..4389102 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
NEB5A_RS21730 4389105..4389446 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
NEB5A_RS21735 4389658..4389909 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
NEB5A_RS21740 4389903..4390253 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
NEB5A_RS21745 4390333..4390863 - 531 WP_000055075.1 inorganic diphosphatase -
NEB5A_RS21750 4391173..4392129 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
NEB5A_RS21755 4392269..4393771 + 1503 WP_000205825.1 sugar ABC transporter ATP-binding protein -
NEB5A_RS21760 4393785..4394807 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T65999 WP_000239577.1 NZ_CP017100:4389903-4390253 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T65999 NZ_CP017100:4389903-4390253 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT65999 WP_001223208.1 NZ_CP017100:4389658-4389909 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT65999 NZ_CP017100:4389658-4389909 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References