65998

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4293019..4293516 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NEB5A_RS21235 Protein ID WP_001173343.1
Coordinates 4293343..4293516 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NEB5A_RS21230 Protein ID WP_000398619.1
Coordinates 4293019..4293315 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS21210 4289750..4290469 - 720 WP_000611288.1 two-component system response regulator DcuR -
NEB5A_RS21215 4290466..4292097 - 1632 WP_001216456.1 sensor histidine kinase -
NEB5A_RS21220 4292278..4292508 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
NEB5A_RS21225 4292520..4292792 + 273 WP_000405651.1 N-acetyltransferase -
NEB5A_RS21230 4293019..4293315 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NEB5A_RS21235 4293343..4293516 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NEB5A_RS21240 4293635..4295152 - 1518 WP_001295074.1 lysine--tRNA ligase -
NEB5A_RS21245 4295389..4296846 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T65998 WP_001173343.1 NZ_CP017100:4293343-4293516 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T65998 NZ_CP017100:4293343-4293516 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT65998 WP_000398619.1 NZ_CP017100:4293019-4293315 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT65998 NZ_CP017100:4293019-4293315 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References