Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2980962..2981616 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NEB5A_RS14815 Protein ID WP_000244777.1
Coordinates 2980962..2981369 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NEB5A_RS14820 Protein ID WP_000354046.1
Coordinates 2981350..2981616 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS14795 2976919..2978652 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
NEB5A_RS14800 2978658..2979368 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NEB5A_RS14805 2979393..2980289 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NEB5A_RS14810 2980401..2980922 + 522 WP_001055874.1 flavodoxin FldB -
NEB5A_RS14815 2980962..2981369 - 408 WP_000244777.1 toxin CptA Toxin
NEB5A_RS14820 2981350..2981616 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NEB5A_RS14825 2981859..2982839 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NEB5A_RS14830 2983035..2983694 - 660 WP_000250274.1 hemolysin III family protein -
NEB5A_RS14835 2983858..2984169 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NEB5A_RS14840 2984214..2985647 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NEB5A_RS14845 2985704..2986447 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T65992 WP_000244777.1 NZ_CP017100:c2981369-2980962 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T65992 NZ_CP017100:c2981369-2980962 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT65992 WP_000354046.1 NZ_CP017100:c2981616-2981350 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT65992 NZ_CP017100:c2981616-2981350 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References