Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2023419..2024250 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag NEB5A_RS10275 Protein ID WP_000854814.1
Coordinates 2023876..2024250 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag NEB5A_RS10270 Protein ID WP_001285584.1
Coordinates 2023419..2023787 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS10255 2021086..2022618 + 1533 WP_001350525.1 hypothetical protein -
NEB5A_RS24720 2022615..2023061 + 447 WP_000187523.1 RadC family protein -
NEB5A_RS10265 2023124..2023345 + 222 WP_000692323.1 DUF987 domain-containing protein -
NEB5A_RS10270 2023419..2023787 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
NEB5A_RS10275 2023876..2024250 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
NEB5A_RS10280 2024247..2024441 + 195 WP_000988600.1 hypothetical protein -
NEB5A_RS25215 2024454..2024567 + 114 WP_001161660.1 DUF957 domain-containing protein -
NEB5A_RS23575 2024856..2024984 - 129 Protein_1972 transposase domain-containing protein -
NEB5A_RS10290 2025056..2025238 + 183 WP_001016348.1 hypothetical protein -
NEB5A_RS10295 2025339..2025668 - 330 WP_000450409.1 DUF496 family protein -
NEB5A_RS10300 2025840..2026898 - 1059 WP_046613327.1 FUSC family protein -
NEB5A_RS10305 2027096..2027569 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
NEB5A_RS10310 2027688..2028854 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T65987 WP_000854814.1 NZ_CP017100:2023876-2024250 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T65987 NZ_CP017100:2023876-2024250 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT65987 WP_001285584.1 NZ_CP017100:2023419-2023787 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT65987 NZ_CP017100:2023419-2023787 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References