Detailed information of TA system TA065987

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2023419..2024250
• Replicon: chromosome
• Accession: NZ_CP017100
• Organism: Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: NEB5A_RS10275
• Protein ID: WP_000854814.1
• Coordinates: 2023876..2024250 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: NEB5A_RS10270
• Protein ID: WP_001285584.1
• Coordinates: 2023419..2023787 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NEB5A_RS10255	2021086..2022618	+	1533	WP_001350525.1	hypothetical protein	-
NEB5A_RS24720	2022615..2023061	+	447	WP_000187523.1	RadC family protein	-
NEB5A_RS10265	2023124..2023345	+	222	WP_000692323.1	DUF987 domain-containing protein	-
NEB5A_RS10270	2023419..2023787	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
NEB5A_RS10275	2023876..2024250	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
NEB5A_RS10280	2024247..2024441	+	195	WP_000988600.1	hypothetical protein	-
NEB5A_RS25215	2024454..2024567	+	114	WP_001161660.1	DUF957 domain-containing protein	-
NEB5A_RS23575	2024856..2024984	-	129	Protein_1972	transposase domain-containing protein	-
NEB5A_RS10290	2025056..2025238	+	183	WP_001016348.1	hypothetical protein	-
NEB5A_RS10295	2025339..2025668	-	330	WP_000450409.1	DUF496 family protein	-
NEB5A_RS10300	2025840..2026898	-	1059	WP_046613327.1	FUSC family protein	-
NEB5A_RS10305	2027096..2027569	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
NEB5A_RS10310	2027688..2028854	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T65987 WP_000854814.1 NZ_CP017100:2023876-2024250 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T65987 NZ_CP017100:2023876-2024250 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT65987 WP_001285584.1 NZ_CP017100:2023419-2023787 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT65987 NZ_CP017100:2023419-2023787 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6