Detailed information of TA system TA065965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 385365..385983
• Replicon: chromosome
• Accession: NZ_CP017100
• Organism: Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: NEB5A_RS01935
• Protein ID: WP_001290581.1
• Coordinates: 385365..385583 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: NEB5A_RS01940
• Protein ID: WP_000344800.1
• Coordinates: 385609..385983 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NEB5A_RS01900	380654..381226	+	573	WP_000779842.1	YbaY family lipoprotein	-
NEB5A_RS01905	381257..381568	-	312	WP_000409911.1	MGMT family protein	-
NEB5A_RS01915	381947..382300	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
NEB5A_RS01920	382342..383892	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
NEB5A_RS01925	384056..384526	-	471	WP_000136192.1	YlaC family protein	-
NEB5A_RS01930	384642..385193	-	552	WP_000102564.1	maltose O-acetyltransferase	-
NEB5A_RS01935	385365..385583	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
NEB5A_RS01940	385609..385983	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
NEB5A_RS01945	386529..389678	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
NEB5A_RS01950	389701..390894	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T65965 WP_001290581.1 NZ_CP017100:c385583-385365 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T65965 NZ_CP017100:c385583-385365 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT65965 WP_000344800.1 NZ_CP017100:c385983-385609 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT65965 NZ_CP017100:c385983-385609 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5