Detailed information of TA system TA065964

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 266566..267245
• Replicon: chromosome
• Accession: NZ_CP017100
• Organism: Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: NEB5A_RS01280
• Protein ID: WP_000854672.1
• Coordinates: 266566..266907 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: NEB5A_RS01285
• Protein ID: WP_000070395.1
• Coordinates: 266928..267245 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NEB5A_RS01255	261664..262872	-	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
NEB5A_RS01260	262979..263338	-	360	Protein_243	porin	-
NEB5A_RS01265	263626..264729	+	1104	WP_001285288.1	glutamate 5-kinase	-
NEB5A_RS01270	264741..265994	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
NEB5A_RS01280	266566..266907	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
NEB5A_RS01285	266928..267245	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
NEB5A_RS22825	267264..267485	-	222	WP_000691994.1	DUF987 domain-containing protein	-
NEB5A_RS01290	267494..267970	-	477	WP_000811693.1	RadC family protein	-
NEB5A_RS01295	267986..268444	-	459	WP_000211838.1	antirestriction protein	-
NEB5A_RS01300	268542..268781	-	240	WP_000194654.1	DUF905 domain-containing protein	-
NEB5A_RS01305	268858..269325	-	468	WP_001547765.1	hypothetical protein	-
NEB5A_RS24990	269348..269791	-	444	WP_000824223.1	hypothetical protein	-
NEB5A_RS24995	269791..270018	-	228	WP_001548158.1	hypothetical protein	-
NEB5A_RS25000	270014..270205	-	192	Protein_255	DeoR family transcriptional regulator	-
NEB5A_RS01320	270422..271243	-	822	WP_000197389.1	DUF945 domain-containing protein	-
NEB5A_RS01325	271335..272198	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T65964 WP_000854672.1 NZ_CP017100:c266907-266566 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T65964 NZ_CP017100:c266907-266566 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT65964 WP_000070395.1 NZ_CP017100:c267245-266928 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT65964 NZ_CP017100:c267245-266928 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684